Montesano Camilla, Vannutelli Gabriele, Gregori Adolfo, Ripani Luigi, Compagnone Dario, Curini Roberta, Sergi Manuel
Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
J Anal Toxicol. 2016 Sep;40(7):519-28. doi: 10.1093/jat/bkw043. Epub 2016 Jul 27.
Drug abuse is today a growing global problem. Often the consumers are not aware about the type of substances they are using and the correlated risks. In recent years, new psychoactive substances (NPS) appeared in the illicit market. The presence of NPS, such as synthetic cathinones, cannabinoids and phenethylamines, which are known to be pharmacologically and toxicologically hazardous, has been frequently reported. The aim of this study was the development of a liquid chromatography-high-resolution mass spectrometry (LC-HRMS) method for a broad screening of NPS in plasma. Data acquisition was in MS/MS and full-scan modes and the method was validated for 25 NPS belonging to different chemical classes. Quantitative results have been obtained for these analytes with limits of quantification ranging from 0.03 to 0.4 ng/mL. The method was proven to be suitable for the screening of additional substances; to this aim, a post-run library matching was conducted for every sample with an in-house database containing over 300 NPS and known metabolites. The library may be constantly expanded with new drugs, in order to obtain a broad screening of NPS in biological matrices.
药物滥用如今是一个日益严重的全球性问题。消费者往往并不知晓他们所使用物质的类型及其相关风险。近年来，新型精神活性物质（NPS）出现在非法市场上。诸如合成卡西酮、大麻素和苯乙胺等NPS的存在，因其在药理学和毒理学上具有危险性，已屡有报道。本研究的目的是开发一种液相色谱 - 高分辨率质谱（LC - HRMS）方法，用于在血浆中广泛筛查NPS。数据采集采用MS/MS和全扫描模式，该方法针对属于不同化学类别的25种NPS进行了验证。已获得这些分析物的定量结果，定量限范围为0.03至0.4 ng/mL。该方法被证明适用于筛查其他物质；为此，对每个样品都与一个包含300多种NPS和已知代谢物的内部数据库进行了运行后库匹配。该库可随着新药不断扩充，以便在生物基质中对NPS进行广泛筛查。
