Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP 13083-859, Brazil.
Campinas Poison Control Center, University of Campinas (UNICAMP), Campinas, SP 13083-859, Brazil.
J Anal Toxicol. 2020 Oct 12;44(7):697-707. doi: 10.1093/jat/bkaa089.
New psychoactive substances (NPS) are a major public health problem, primarily due to the increased number of acute poisoning cases. Detection of these substances is a challenge. The aim of this research was to develop and validate a sensitive screening method for 104 drugs of abuse, including synthetic cannabinoids, synthetic cathinones, fentanyl analogues, phenethylamines and other abused psychoactive compounds (i.e., THC, MDMA, LSD and their metabolites) in oral fluid by liquid chromatography-tandem mass spectrometry (LC-MS-MS). The Quantisal™ oral fluid device was used to collect oral fluid samples. The oral fluid-elution buffer mixture (500-μL sample) was extracted with t-butyl methyl ether, and chromatographic separation was performed on a Raptor™ biphenyl column (100 × 2.1 mm ID, 2.7 μm), with a total run time of 13.5 min. Limits of detection were established at three concentrations (0.05, 0.1 or 1 ng/mL) for most analytes, except for acetyl norfentanyl and mescaline (5 ng/mL). Matrix effects were generally <20% and overall extraction recoveries >60%. The highest matrix effect was observed within the synthetic cannabinoid group (PB22, -55.5%). Lower recoveries were observed for 2C-T (47.2%) and JWH-175 (58.7%). Recoveries from the Quantisal™ device were also evaluated for all analytes (56.7-127%), with lower recoveries noted for 25I-NBOMe, valerylfentanyl and mCPP (56.7, 63.0 and 69.9%, respectively). Drug stability in oral fluid was evaluated at 15, 60 and 90 days and at 25, 4 and -20°C. As expected, greater stability was observed when samples were stored at -20°C, but even when frozen, some NPS (e.g., synthetic cannabinoids) showed more than 20% degradation. The method was successfully applied to the analysis of seven authentic oral fluid samples positive for 17 different analytes. The method achieved good sensitivity and simultaneous detection of a wide range of NPS.
新型精神活性物质（NPS）是一个主要的公共卫生问题，主要是因为急性中毒病例的数量增加。这些物质的检测是一个挑战。本研究的目的是开发和验证一种灵敏的筛选方法，用于检测 104 种药物滥用物质，包括合成大麻素、合成卡西酮、芬太尼类似物、苯乙胺和其他滥用精神活性化合物（即 THC、MDMA、LSD 及其代谢物）在口服液中的含量，采用液相色谱-串联质谱法（LC-MS-MS）。Quantisal™ 口腔液采集装置用于采集口腔液样本。将口腔液洗脱缓冲液混合物（500 μL 样品）用叔丁基甲基醚提取，采用 Raptor™ 联苯柱（100×2.1mm ID，2.7μm）进行色谱分离，总运行时间为 13.5min。除乙酰基去甲芬太尼和三甲氧苯乙胺（5ng/mL）外，大多数分析物在三个浓度（0.05、0.1 或 1ng/mL）下建立了检测限。基质效应一般小于 20%，总体提取回收率大于 60%。在合成大麻素组中观察到最高的基质效应（PB22，-55.5%）。2C-T（47.2%）和 JWH-175（58.7%）的回收率较低。还评估了 Quantisal™ 装置对所有分析物的回收率（56.7-127%），25I-NBOMe、伐仑氟太尼和 mCPP 的回收率较低（分别为 56.7%、63.0%和 69.9%）。在 15、60 和 90 天以及 25、4 和-20°C 条件下评估了口服液中的药物稳定性。正如预期的那样，当样品储存在-20°C 时，稳定性更高，但即使在冷冻条件下，一些 NPS（如合成大麻素）也会发生超过 20%的降解。该方法成功应用于 7 个真实的口腔液样本中 17 种不同分析物的分析。该方法具有良好的灵敏度和对广泛的 NPS 的同时检测能力。
