Liu Jinlong, Fu Shaomin, Yang Lei, Luan Mingda, Zhao Fugeng, Luan Sheng, Lan Wenzhi
a State Key Laboratory for Pharmaceutical Biotechnology, Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, College of Life Sciences, Nanjing University , Nanjing , China.
b Department of Plant and Microbial Biology , University of California , Berkeley , CA , USA.
Plant Signal Behav. 2016 Aug 2;11(8):e1213474. doi: 10.1080/15592324.2016.1213474.
To survive in most soils in which inorganic phosphate (Pi) levels are limited and constantly changing, plants universally use the vacuoles as cellular Pi "sink" and "source" to maintain Pi homeostasis. However, the transporters that mediate Pi sequestration into the vacuoles remain unknown. Recently, we and other 2 groups independently identified the members of SPS-MSF family as the candidates for tonoplast Pi transporters in Arabidopsis thaliana and Oryza sativa. We and Liu et al. demonstrated that one of SPS-MSF member, VPT1 (Vacuolar Phosphate Transporter 1), also named as PHT5;1 (Phosphate Transporter 5;1), plays a predominant role in Pi sequestration of vacuoles in Arabidopsis. Here we show that vpt1 mutants and VPT1-GFP overexpressing lines displayed sensitive to Pi stress under the hydroponic system containing the medium with low iron, supporting that VPT1 is essential for Arabidopsis to adapt phosphate stress.
为了在大多数无机磷酸盐（Pi）水平有限且不断变化的土壤中生存，植物普遍将液泡用作细胞内Pi的“汇”和“源”，以维持Pi的稳态。然而，介导Pi隔离到液泡中的转运蛋白仍然未知。最近，我们和其他两个研究小组独立鉴定出SPS-MSF家族成员是拟南芥和水稻中液泡膜Pi转运蛋白的候选者。我们和Liu等人证明，SPS-MSF成员之一，VPT1（液泡磷酸盐转运蛋白1），也被命名为PHT5;1（磷酸盐转运蛋白5;1），在拟南芥液泡的Pi隔离中起主要作用。在这里我们表明，在含有低铁培养基的水培系统中，vpt1突变体和VPT1-GFP过表达系对Pi胁迫敏感，这支持了VPT1对拟南芥适应磷胁迫至关重要。
