Genome-Wide Analysis of DoSPX Genes and the Function of in Low Phosphorus Response in .

Kimura et Migo is a famous Chinese herb. grows on rocks where the available phosphorus is low. The SPX family plays a critical role in maintaining Pi homeostasis in plants. In this paper, 9 SPX family genes were identified in the genome of . Bioinformatics and qRT-PCR analysis showed that DoSPXs were involved in response to -Pi stress and had different expression patterns. , which had a unique expression pattern, was clustered with AtSPX4 and OsSPX4. Under -Pi treatment, the expression level of reached a peak on 5 d in roots, while showing a downward trend in the aboveground parts. DoSPX4 was located on the cell membrane. Overexpression promoted Pi content in the stem and the expression level of in . The results of Yeast two-hybrid showed that DoSPX4 could interact with Phosphate High-Affinity Response factor (DoPHR2). These results highlight the role of in response to low phosphorus, which provides a theoretical basis for further study on the response mechanism of -Pi in .