Liu Lin, Xiang Haoxin, Song Jingjing, Shen Huimin, Sun Xu, Tian Lingfeng, Fan Honghong
School of Life Sciences, Anhui Agricultural University, Hefei, China.
Front Plant Sci. 2022 Jul 11;13:943788. doi: 10.3389/fpls.2022.943788. eCollection 2022.
Kimura et Migo is a famous Chinese herb. grows on rocks where the available phosphorus is low. The SPX family plays a critical role in maintaining Pi homeostasis in plants. In this paper, 9 SPX family genes were identified in the genome of . Bioinformatics and qRT-PCR analysis showed that DoSPXs were involved in response to -Pi stress and had different expression patterns. , which had a unique expression pattern, was clustered with AtSPX4 and OsSPX4. Under -Pi treatment, the expression level of reached a peak on 5 d in roots, while showing a downward trend in the aboveground parts. DoSPX4 was located on the cell membrane. Overexpression promoted Pi content in the stem and the expression level of in . The results of Yeast two-hybrid showed that DoSPX4 could interact with Phosphate High-Affinity Response factor (DoPHR2). These results highlight the role of in response to low phosphorus, which provides a theoretical basis for further study on the response mechanism of -Pi in .
金铁锁是一种著名的中药材。生长在有效磷含量低的岩石上。SPX家族在维持植物磷稳态中起关键作用。本文在[植物名称]基因组中鉴定出9个SPX家族基因。生物信息学和qRT-PCR分析表明，DoSPXs参与了对低磷胁迫的响应且具有不同的表达模式。[基因名称]具有独特的表达模式，与拟南芥AtSPX4和水稻OsSPX4聚类。在低磷处理下，[基因名称]在根中的表达水平在第5天达到峰值，而在地上部分呈下降趋势。DoSPX4定位于细胞膜。[基因名称]过表达促进了茎中磷含量以及[植物名称]中[基因名称]的表达水平。酵母双杂交结果表明，DoSPX4可与磷酸盐高亲和力响应因子（DoPHR2）相互作用。这些结果突出了[基因名称]在响应低磷中的作用，为进一步研究[植物名称]对低磷的响应机制提供了理论依据。
