Tabatadze Nino, Woolley Catherine
Department of Neurobiology, Northwestern University, Evanston, USA.
Bio Protoc. 2016 Apr 5;6(7). doi: 10.21769/bioprotoc.1780.
Inositol triphosphate (IP) is an important second messenger that participates in signal transduction pathways in diverse cell types including hippocampal neurons. Stimulation of phospholipase C in response to various stimuli (hormones, growth factors, neurotransmitters, neurotrophins, neuromodulators, odorants, light, ) results in hydrolysis of phosphatidylinositol 4, 5-bisphosphate (PIP), a phospholipid that is located in the plasma membrane, and leads to the production of IP and diacylglycerol. Binding of IP to the IP receptor (IPR) induces Ca release from intracellular stores and enables the initiation of intracellular Ca-dependent signaling. Here we describe a procedure for the measurement of cellular IP levels in tissue homogenates prepared from rat hippocampal slices.
肌醇三磷酸（IP）是一种重要的第二信使，参与包括海马神经元在内的多种细胞类型的信号转导途径。响应各种刺激（激素、生长因子、神经递质、神经营养因子、神经调质、气味剂、光等）刺激磷脂酶C，会导致位于质膜中的磷脂酰肌醇4,5-二磷酸（PIP）水解，并产生IP和二酰基甘油。IP与IP受体（IPR）结合会诱导细胞内储存库释放Ca，并启动细胞内Ca依赖性信号传导。在此，我们描述了一种测量从大鼠海马切片制备的组织匀浆中细胞IP水平的方法。
