College of Life Science and Engineering, Northwest Minzu University, Lanzhou, Gansu 730030, China.
State Engineering Technology Institute for Karst Desertification Control, Guizhou Normal University, Guiyang, Guizhou 550001, China.
Biomed Res Int. 2018 Jul 9;2018:5032875. doi: 10.1155/2018/5032875. eCollection 2018.
Mice of FRBI-1, FRBI-2, and FRBI-3 groups were intramuscularly injected with 20, 30, and 40mg/kg, respectively, for five consecutive days. Ovarian weights of three FRBI groups were reduced in comparison with FSH group. Ovarian cortex thicknesses (OCT) of the FRBI-3 group were less than that of the FSH group (P<0.05). As compared to FSH group, there were fewer numbers of secondary follicles (SFs) and mature follicles (MF) on the ovaries of FRBI-treated mice numbers of primary follicles (PFs) and SFs also decreased. In FRBI-3 mice, we found that the primordial follicles (POF) were scarcer, the follicles developed poorly, and granulosa cells became apoptosis. SF numbers of FRBI-2 and FRBI-3 groups were less than that of the FSH group on day 20 (P<0.05). Maximum longitudinal diameter (MLD) and transverse diameter (MTD) of three FRBI groups became decreased during the experiment. MLD and MTD of the FRBI-3 group were smaller than FSH group. Levels of FSHR mRNA and protein were less than that of CG and FSH group (P<0.05). ER protein levels of FRBI group and serum concentrations of FSH and estradiol (E) in the FRBI-treated mice were decreased when compared to CG and FSH group. In conclusion, FSH treatment could increase the numbers of SF and MF, enhance follicle development, reduce the numbers of SF and MF, and depress the follicular development of mice. Furthermore, FRBI declined the mRNA and protein levels of ER and FSHR in the ovaries and dropped serum concentrations of FSH and E of mice.
FRBI-1、FRBI-2 和 FRBI-3 组的小鼠分别肌肉注射 20、30 和 40mg/kg，连续 5 天。与 FSH 组相比，FRBI 三组的卵巢重量降低。FRBI-3 组的卵巢皮质厚度（OCT）低于 FSH 组（P<0.05）。与 FSH 组相比，FRBI 处理组小鼠卵巢上的次级卵泡（SF）和成熟卵泡（MF）数量减少，初级卵泡（PF）和 SF 数量也减少。在 FRBI-3 小鼠中，我们发现原始卵泡（POF）较少，卵泡发育不良，颗粒细胞凋亡。FRBI-2 和 FRBI-3 组在第 20 天的 SF 数量少于 FSH 组（P<0.05）。三个 FRBI 组的最大纵向直径（MLD）和横向直径（MTD）在实验过程中均降低。FRBI-3 组的 MLD 和 MTD 小于 FSH 组。FRBI 组的 FSHR mRNA 和蛋白水平低于 CG 和 FSH 组（P<0.05）。与 CG 和 FSH 组相比，FRBI 组的 ER 蛋白水平和血清中 FSH 和雌二醇（E）的浓度降低。总之，FSH 处理可增加 SF 和 MF 的数量，增强卵泡发育，减少 SF 和 MF 的数量，并抑制小鼠卵泡发育。此外，FRBI 降低了卵巢中 ER 和 FSHR 的 mRNA 和蛋白水平，并降低了小鼠血清中 FSH 和 E 的浓度。
