Yang Lu-lu, Ma Lin, Wang Hong, Chen Ke-fei, Lu Qiang, Ling Wen-wu, Luo Yan
Sichuan Da Xue Xue Bao Yi Xue Ban. 2016 May;47(3):326-30.
To explore portal vein hemodynamic changes and liver regeneration in rats after 70% partial hepatectomy (PH).
Rats were randomly subjected into sham operation group and 70% PH group. A 5-12 MHz high-frequency linear transducer was applied to measure the portal vein diameter (PVD) and the maximum portal vein velocity (PVV) before and after operation on day 1, 3, 7, 14. On the corresponding time, liver tissue morphological changes were observed, and the expression level of PCNA was measured, and the liver regeneration rate (LRR) was calculated.
On day 1 after PH, PCNA began to increase, and hepatocyte vacuolated obviously which squeezed and narrowed the hepatic sinusoid. Meanwhile, PVD started to dilate and PVV decelerated. On day 3 after operation, PCNA peaked and active mitosis resulted in the disorders of hepatic structure. PVD peaked and PVV decelerated to the lowest. Until the 14 d after operation, the cell morphology, lobular structure and PCNA recovered gradually accompany by LRR above 90%. PVD and PVV also almost recovered to levels closed to sham group (P < 0.05).
The hemodynamic parameters PVD, PVV correlated with hepatic pathology changes, mitosis status and regenerated liver volume after PH, which state the potential possibility for ultrasound image study in liver regeneration surveillance.
探讨70%肝部分切除术（PH）后大鼠门静脉血流动力学变化及肝再生情况。
将大鼠随机分为假手术组和70% PH组。于术后第1、3、7、14天，应用5-12 MHz高频线性探头测量门静脉直径（PVD）和门静脉最大血流速度（PVV）。在相应时间观察肝组织形态学变化，检测增殖细胞核抗原（PCNA）表达水平，并计算肝再生率（LRR）。
PH术后第1天，PCNA开始升高，肝细胞明显空泡化，挤压并使肝血窦变窄。同时，PVD开始扩张，PVV减慢。术后第3天，PCNA达到峰值，活跃的有丝分裂导致肝结构紊乱。PVD达到峰值，PVV减慢至最低。至术后14天，细胞形态、小叶结构及PCNA逐渐恢复，LRR高于90%。PVD和PVV也几乎恢复至接近假手术组的水平（P < 0.05）。
血流动力学参数PVD、PVV与PH术后肝脏病理变化、有丝分裂状态及再生肝体积相关，这表明超声图像研究在肝再生监测中的潜在可能性。
