Wang Ling, Huang Xue-kuan, Wan Lei, Yang Yu
Sichuan Da Xue Xue Bao Yi Xue Ban. 2016 May;47(3):342-6.
To investigate the effect of Fushengong Decoction on the expression of nephrin mRNA in renal tissue of rats with chronic renal failure (CRF).
Fifty five male SD rats were randomly divided into five groups: control group, CRF model group, and low, medium and high Fushengong Decoction dose groups. Rats in control group were fed with standard chow, while the other four groups were fed with adenine to make CRF. The rats in control group received intra-gastric normal saline (NS) of 20 mL/(kg · d) for 30 d, while those in low, medium and high Fushengong Decoction dose groups received Fushengong Decoction at the dose of 4 g/kg, 8 g/kg and 16 g/kg respectively, once a day for 30 d. After that, 24 h urinary protein in urine was measured, blood urea nitrogen (BUN) and serum creatinine (SCr) were detected. Histomorphology of glomerulus were studied by HE staining, and the expression of nephrin were detected by immunohistochemistry and RT-PCR.
Compared to the control group, the levels of 24 h urinary protein, BUN and SCr increased significantly (P < 0.05) and the expression of nephrin protein and mRNA decreased significantly (P < 0.05) in CRF model group. The renal interstitium showed fibrotic lesions in model group. The levels of 24 h urinary protein, BUN and SCr decreased significantly after the treatment of Fushengong Decoction (P < 0.05), while the expression of nephrin protein and mRNA increased significantly (P < 0.05).
Fushengong Decoction could reduce urinary protein and relieve renal damage in rats with CRF by improving the expression of nephrin and reducing the injury of podocytes.
探讨复肾功汤对慢性肾衰竭（CRF）大鼠肾组织中nephrin mRNA表达的影响。
将55只雄性SD大鼠随机分为5组：对照组、CRF模型组、复肾功汤低、中、高剂量组。对照组大鼠给予标准饲料，其余4组大鼠给予腺嘌呤制备CRF模型。对照组大鼠给予20 mL/(kg·d)的生理盐水灌胃30天，复肾功汤低、中、高剂量组大鼠分别给予4 g/kg、8 g/kg、16 g/kg的复肾功汤灌胃，每日1次，共30天。之后，检测24小时尿蛋白，测定血尿素氮（BUN）和血清肌酐（SCr）。采用HE染色观察肾小球组织形态学变化，免疫组化和RT-PCR检测nephrin的表达。
与对照组相比，CRF模型组大鼠24小时尿蛋白、BUN和SCr水平显著升高（P<0.05），nephrin蛋白和mRNA表达显著降低（P<0.05）。模型组肾间质出现纤维化病变。复肾功汤治疗后，大鼠24小时尿蛋白、BUN和SCr水平显著降低（P<0.05），nephrin蛋白和mRNA表达显著升高（P<0.05）。
复肾功汤可通过提高nephrin表达、减轻足细胞损伤，降低CRF大鼠尿蛋白水平，减轻肾脏损害。
