Yang Yu, Wei Junmeng, Huang Xuekuan, Wu Mingjun, Lv Zhenbing, Tong Pan, Chang Rui
College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400016, China.
Department of Pathogenic Biology, Chongqing Medical University, Chongqing 400016, China.
Evid Based Complement Alternat Med. 2017;2017:1480514. doi: 10.1155/2017/1480514. Epub 2017 Apr 27.
. Chronic renal failure (CRF) has become a global health problem and bears a huge economic burden. FuShengong Decoction (FSGD) as traditional Chinese medicine has multiple pharmacological effects. . To understand the underlying molecular mechanism and signaling pathway involved in the FSGD treatment of CRF and screen differentially expressed proteins in rats with CRF treated with FSGD. . Thirty-three male Sprague-Dawley rats were randomly divided into control group, CRF group, and FSGD group. Differentially expressed proteins were screened by iTRAQ coupled with nanoLC-MS/MS, and these identified proteins were later analyzed by GO, KEGG, and STRING. Additionally, haptoglobin (HP) and alpha-1-antitrypsin (AAT) were finally verified by ELISA, Western blot, and real time PCR. . A total of 417 proteins were identified. Nineteen differentially expressed proteins were identified in the FSGD group compared with the model group, of which 3 proteins were upregulated and 16 proteins were downregulated. Cluster analysis indicated that inflammatory response was associated with these proteins and complement and coagulation cascade pathways were predominantly involved. The validation methods further confirmed that the levels of HP and AAT were significantly increased. . HP and AAT may be the important biomarkers in the pathogenesis of CRF and FSGD therapy.
慢性肾衰竭（CRF）已成为一个全球性的健康问题，并带来了巨大的经济负担。中药复肾功汤（FSGD）具有多种药理作用。为了解FSGD治疗CRF的潜在分子机制和信号通路，并筛选FSGD治疗的CRF大鼠中差异表达的蛋白质。将33只雄性Sprague-Dawley大鼠随机分为对照组、CRF组和FSGD组。通过iTRAQ结合nanoLC-MS/MS筛选差异表达蛋白质，随后对这些鉴定出的蛋白质进行GO、KEGG和STRING分析。此外，最后通过ELISA、Western印迹和实时PCR对触珠蛋白（HP）和α-1抗胰蛋白酶（AAT）进行验证。共鉴定出417种蛋白质。与模型组相比，FSGD组鉴定出19种差异表达蛋白质，其中3种蛋白质上调，16种蛋白质下调。聚类分析表明，炎症反应与这些蛋白质相关，主要涉及补体和凝血级联途径。验证方法进一步证实HP和AAT水平显著升高。HP和AAT可能是CRF发病机制和FSGD治疗中的重要生物标志物。
