环孢素A诱导的牙龈过度增生中谷氨酰胺转移酶-2及氧化应激标志物在龈沟液和血浆中的水平

Gingival Crevicular Fluid and Plasma Levels of Transglutaminase-2 and Oxidative Stress Markers in Cyclosporin A-Induced Gingival Overgrowth.

作者信息

Becerik Sema, Celec Peter, Gürkan Ali, Öztürk Veli Özgen, Kamodyova Natalia, Atilla Gül, Emingil Gülnur

机构信息

Department of Periodontology, School of Dentistry, Ege University, İzmir, Turkey.

Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia.

出版信息

J Periodontol. 2016 Dec;87(12):1508-1516. doi: 10.1902/jop.2016.160031. Epub 2016 Jul 29.

DOI:10.1902/jop.2016.160031

PMID:27468796

Abstract

BACKGROUND

Transglutaminase (TGM)-2 has been shown to contribute to fibrosis by extracellular matrix accumulation in some organs and is activated by intracellular reactive oxygen species. The aim of this study is to investigate levels of gingival crevicular fluid (GCF) and plasma TGM-2 and oxidative stress markers (OSMs) in cyclosporin A (CsA)-induced gingival overgrowth (GO).

METHODS

The study enrolled 20 healthy (H) individuals; 20 patients with gingivitis (G); 20 CsA-medicated patients with GO (CsA GO+); and 20 CsA-medicated patients without GO (CsA GO-). GCF and plasma levels of TGM-2 were analyzed by enzyme-linked immunosorbent assay. Spectrofluorometry was used to analyze thiobarbituric acid reactive substance (TBARS); ferric-reducing antioxidant power (FRAP); total oxidant status (TOS); and total antioxidant capacity (TAC).

RESULTS

GCF TGM-2 level was elevated in CsA GO+ compared with G (P = 0.048) and H (P = 0.001) groups. GCF TBARS level was elevated in CsA GO+ compared with other groups (CsA GO- group: P = 0.003; G group: P <0.001; and H group: P <0.001) and was higher in CsA GO- than in H (P = 0.048). GCF FRAP level was lower in CsA GO- than in H (P = 0.04). Both CsA GO+ and CsA GO- groups had lower GCF TOS levels than H (P <0.001 and P = 0.002) and G (P = 0.003 and P = 0.04). GCF TAC was higher in CsA GO+ than in H (P = 0.02). Plasma TGM-2 level was elevated in CsA GO+ compared with G (P = 0.048) and H (P = 0.002). Plasma FRAP level was higher in H and CsA GO- than in CsA GO+ (P = 0.008 and P = 0.02).

CONCLUSIONS

CsA use significantly alters GCF and plasma levels of TGM-2 and OSMs. TGM-2 may contribute to CsA-induced GO in CsA-treated patients by changing GCF and plasma levels of OSMs. Further studies are needed to prove causality and its direction.

摘要

背景

转谷氨酰胺酶（TGM）-2已被证明可通过某些器官中的细胞外基质积累促进纤维化，并被细胞内活性氧激活。本研究的目的是调查环孢素A（CsA）诱导的牙龈过度生长（GO）中龈沟液（GCF）和血浆TGM-2以及氧化应激标志物（OSM）的水平。

方法

该研究纳入了20名健康（H）个体；20名牙龈炎患者（G）；20名服用CsA的GO患者（CsA GO+）；以及20名服用CsA但无GO的患者（CsA GO-）。通过酶联免疫吸附测定法分析GCF和血浆中TGM-2的水平。采用分光荧光法分析硫代巴比妥酸反应性物质（TBARS）；铁还原抗氧化能力（FRAP）；总氧化剂状态（TOS）；以及总抗氧化能力（TAC）。

结果

与G组（P = 0.048）和H组（P = 0.001）相比，CsA GO+组的GCF TGM-2水平升高。与其他组相比，CsA GO+组的GCF TBARS水平升高（CsA GO-组：P = 0.003；G组：P <0.001；H组：P <0.001），且CsA GO-组高于H组（P = 0.048）。CsA GO-组的GCF FRAP水平低于H组（P = 0.04）。CsA GO+组和CsA GO-组的GCF TOS水平均低于H组（P <0.001和P = 0.002）以及G组（P = 0.003和P = 0.04）。CsA GO+组的GCF TAC高于H组（P = 0.02）。与G组（P = 0.048）和H组（P = 0.002）相比，CsA GO+组的血浆TGM-2水平升高。H组和CsA GO-组的血浆FRAP水平高于CsA GO+组（P = 0.008和P = 0.02）。