Mojsilović Jana, Jovičić Nemanja, Vujović Ristić Sanja, Stevanović Momir, Mijailović Sara, Rosić Gvozden, Janković Slobodan, Kostić Marina
Department of Dentistry, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, Serbia.
Department of Histology and Emrbiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, Serbia.
Pharmaceuticals (Basel). 2024 Aug 16;17(8):1075. doi: 10.3390/ph17081075.
This study aimed to identify risk factors for amlodipine-induced gingival enlargement, assess quality of life, and analyze gingival tissue. This cross-sectional study involved hypertensive patients on amlodipine, divided into groups with and without gingival enlargement. Assessments included sociodemographic data, clinical evaluations, and clinical parameters. Quality of life was assessed using OHIP-14 and WB-HRQoL scales. Gingival tissue samples were analyzed for oxidative status and key molecules using RT-PCR and colorimetric assays. The study included 32 patients with no significant sociodemographic differences between groups ( > 0.05). Patients with gingival enlargement had higher systolic blood pressure (139.63 ± 10.743 vs. 128.38 ± 7.249, = 0.028) and higher OHIP-14 scores. The RT-PCR analysis showed significant differences in IL-6, TNF-α, IL-33, ST2, TGF-β1, FGF-2, CTGF, VEGF-D, and KGF expression. IL-6, TNF-α, ST2, and FGF-2 expression levels were lower in patients taking amlodipine, with and without gingival enlargement. TGF-β1 and CTGF expression levels were highest in patients with amlodipine-induced gingival enlargement. SOD activity was also highest in these patients, whereas MDA levels were higher in patients with gingival enlargement without amlodipine. Our study highlights the impact of amlodipine-induced gingival enlargement on oral health and quality of life, emphasizing fibrosis and oxidative stress, and suggests the need for integrated healthcare approaches and further research.
本研究旨在确定氨氯地平所致牙龈增生的危险因素，评估生活质量，并分析牙龈组织。这项横断面研究纳入了服用氨氯地平的高血压患者，分为有牙龈增生组和无牙龈增生组。评估内容包括社会人口统计学数据、临床评估和临床参数。使用OHIP-14和WB-HRQoL量表评估生活质量。采用逆转录聚合酶链反应（RT-PCR）和比色法分析牙龈组织样本的氧化状态和关键分子。该研究纳入了32例患者，两组间社会人口统计学差异无统计学意义（>0.05）。牙龈增生患者的收缩压较高（139.63±10.743 vs. 128.38±7.249，P=0.028）且OHIP-14评分较高。RT-PCR分析显示白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、白细胞介素-33（IL-33）、ST2、转化生长因子-β1（TGF-β1）、成纤维细胞生长因子-2（FGF-2）、结缔组织生长因子（CTGF）、血管内皮生长因子-D（VEGF-D）和角质形成细胞生长因子（KGF）表达存在显著差异。无论有无牙龈增生，服用氨氯地平患者的IL-6、TNF-α、ST2和FGF-2表达水平均较低。TGF-β1和CTGF表达水平在氨氯地平所致牙龈增生患者中最高。这些患者的超氧化物歧化酶（SOD）活性也最高，而无氨氯地平的牙龈增生患者的丙二醛（MDA）水平较高。我们的数据突出了氨氯地平所致牙龈增生对口腔健康和生活质量的影响，强调了纤维化和氧化应激，并表明需要综合医疗保健方法和进一步研究。
