Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore.
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore; NUS Environmental Research Institute (NERI), #02-01, T-Lab Building (TL), 5A Engineering Drive 1, Singapore 117411, Singapore.
Bioresour Technol. 2016 Oct;218:1115-22. doi: 10.1016/j.biortech.2016.07.079. Epub 2016 Jul 21.
In this study, (1)H nuclear magnetic resonance (NMR)-based metabolomics approach was used to characterize the metabolic response of the earthworm Perionyx excavatus in continuous vermifiltration for two months under hydraulic loading rates of 1m(3)m(-2)d(-1) (VF1) and 1.5m(3)m(-2)d(-1) (VF1.5). Both VF1 and VF1.5 showed higher removal of chemical oxygen demand and total nitrogen than the biofilter without earthworms. Principal component analysis of the NMR spectra of earthworm metabolites showed significant separations between those not subjected to wastewater filtration (control) and VF1 or VF1.5. Temporal variations of earthworm biomass, and the identified metabolites that are significantly different between control, VF1 and VF1.5 revealed that worms underwent increasing metabolic activity within 20days in VF1 and 14days in VF1.5, then decreasing metabolic activity. The use of NMR-based metabolomics in monitoring earthworm metabolism was demonstrated to be a novel approach in studying engineered vermifiltration systems.
在这项研究中，采用基于 1H 核磁共振（NMR）的代谢组学方法来表征蚯蚓 Perionyx excavatus 在水力负荷率为 1m3m-2d-1（VF1）和 1.5m3m-2d-1（VF1.5）的连续蚯蚓过滤系统中连续两个月的代谢反应。VF1 和 VF1.5 均表现出比无蚯蚓的生物滤池更高的化学需氧量和总氮去除率。未经过废水过滤的（对照）与 VF1 或 VF1.5 的蚯蚓代谢物 NMR 图谱的主成分分析显示出明显的分离。蚯蚓生物量和在对照、VF1 和 VF1.5 之间存在显著差异的鉴定代谢物的时间变化表明，在 VF1 中蚯蚓在 20 天内经历了代谢活性的增加，在 VF1.5 中则在 14 天内经历了代谢活性的增加，然后代谢活性下降。证明了基于 NMR 的代谢组学在监测蚯蚓代谢中的应用是研究工程化蚯蚓过滤系统的一种新方法。
