基因组编辑工具CRISPR/Cas9在非人灵长类动物中的应用。
Application of the genome editing tool CRISPR/Cas9 in non-human primates.
作者信息
Luo Xin, Li Min, Su Bing
机构信息
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China;Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, Chin.
出版信息
Dongwuxue Yanjiu. 2016 Jul 18;37(4):214-9. doi: 10.13918/j.issn.2095-8137.2016.4.214.
Abstract
In the past three years, RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system has been used to facilitate efficient genome editing in many model and non-model animals. However, its application in nonhuman primates is still at the early stage, though in view of the similarities in anatomy, physiology, behavior and genetics, closely related nonhuman primates serve as optimal models for human biology and disease studies. In this review, we summarize the current proceedings of gene editing using CRISPR/Cas9 in nonhuman primates.
摘要
在过去三年中，来自微生物成簇规律间隔短回文重复序列（CRISPR）适应性免疫系统的RNA引导的Cas9核酸酶已被用于促进许多模式动物和非模式动物的高效基因组编辑。然而，其在非人灵长类动物中的应用仍处于早期阶段，尽管鉴于在解剖学、生理学、行为和遗传学方面的相似性，亲缘关系较近的非人灵长类动物是人类生物学和疾病研究的最佳模型。在这篇综述中，我们总结了目前在非人灵长类动物中使用CRISPR/Cas9进行基因编辑的进展。
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