Chen Lei, Wang Gui, Zhu Ya-Nan, Xiang Hui, Wang Wen
State Key Laboratory of Genetic Resources & Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China;University of Chinese Academy of Sciences, Beijing 100049, China.
College of Hetao, Bayannaoer Inner Mongolia 015000, China.
Dongwuxue Yanjiu. 2016 Jul 18;37(4):220-8. doi: 10.13918/j.issn.2095-8137.2016.4.220.
Insects compose more than half of all living organisms on earth, playing essential roles in global ecosystems and forming complex relationships with humans. Insect research has significant biological and practical importance. However, the application of genetic manipulation technology has long been restricted to several model insects only, such as gene knockout in Drosophila, which has severely restrained the development of insect biology research. Recently, with the increase in the release of insect genome data and the introduction of the CRISPR/Cas9 system for efficient genetic modification, it has been possible to conduct meaningful functional studies in a broad array of insect species. Here, we summarize the advances in CRISPR/Cas9 in different insect species, discuss methods for its promotion, and consider its application in future insect studies. This review provides detailed information about the application of the CRISPR/Cas9 system in insect research and presents possible ways to improve its use in functional studies and insect pest control.
昆虫占地球上所有生物的一半以上，在全球生态系统中发挥着重要作用，并与人类形成复杂的关系。昆虫研究具有重大的生物学和实际意义。然而，基因操作技术的应用长期以来仅限于几种模式昆虫，如在果蝇中进行基因敲除，这严重限制了昆虫生物学研究的发展。最近，随着昆虫基因组数据的不断发布以及用于高效基因编辑的CRISPR/Cas9系统的引入，已经能够在广泛的昆虫物种中进行有意义的功能研究。在此，我们总结了CRISPR/Cas9在不同昆虫物种中的研究进展，讨论了其推广方法，并考虑了其在未来昆虫研究中的应用。本综述提供了有关CRISPR/Cas9系统在昆虫研究中应用的详细信息，并提出了在功能研究和害虫防治中改进其应用的可能方法。
