Zhang Xiao-Liang, Song Jia-Hao, Pang Wei, Zheng Yong-Tang
Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China;Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650500, China.
Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China;Institute of Health Sciences, Anhui University, Hefei Anhui 230601, China.
Dongwuxue Yanjiu. 2016 Jul 18;37(4):246-51. doi: 10.13918/j.issn.2095-8137.2016.4.246.
Northern pig-tailed macaques (NPMs, Macaca leonina) are susceptible to HIV-1 infection largely due to the loss of HIV-1-restricting factor TRIM5α. However, great impediments still exist in the persistent replication of HIV-1 in vivo, suggesting some viral restriction factors are reserved in this host. The APOBEC3 proteins have demonstrated a capacity to restrict HIV-1 replication, but their inhibitory effects in NPMs remain elusive. In this study, we cloned the NPM A3A-A3H genes, and determined by BLAST searching that their coding sequences (CDSs) showed 99% identity to the corresponding counterparts from rhesus and southern pig-tailed macaques. We further analyzed the anti-HIV-1 activities of the A3A-A3H genes, and found that A3G and A3F had the greatest anti-HIV-1 activity compared with that of other members. The results of this study indicate that A3G and A3F might play critical roles in limiting HIV-1 replication in NPMs in vivo. Furthermore, this research provides valuable information for the optimization of monkey models of HIV-1 infection.
北豚尾猕猴（NPMs，狮尾猕猴）易受HIV-1感染，主要原因是失去了限制HIV-1的因子TRIM5α。然而，HIV-1在体内持续复制仍存在很大障碍，这表明该宿主中仍保留一些病毒限制因子。载脂蛋白B mRNA编辑酶催化多肽样3（APOBEC3）蛋白已显示出限制HIV-1复制的能力，但其在北豚尾猕猴中的抑制作用仍不清楚。在本研究中，我们克隆了北豚尾猕猴的A3A - A3H基因，并通过BLAST搜索确定其编码序列（CDS）与恒河猴和南豚尾猕猴的相应序列具有99%的同一性。我们进一步分析了A3A - A3H基因的抗HIV-1活性，发现与其他成员相比，A3G和A3F具有最强的抗HIV-1活性。本研究结果表明，A3G和A3F可能在限制HIV-1在北豚尾猕猴体内复制中起关键作用。此外，本研究为优化HIV-1感染的猴模型提供了有价值的信息。
