INFIQC, Centro Laser de Ciencias Moleculares, Departamento de Fisicoquímica, Córdoba, 5000, Argentina.
Nanoscale. 2016 Oct 21;8(39):17169-17180. doi: 10.1039/c6nr04897h. Epub 2016 Jul 29.
To date, while various diagnostic approaches for antigen detection have been proposed, most are too expensive, lengthy and limited in sensitivity for clinical use. Nanoparticle systems with unique material properties, however, circumvent these problems and offer improved accuracy and sensitivity over current methods like the enzyme-linked immunosorbent assay (ELISA). Herein, we present a novel functionalization strategy of plasmonic nanoparticle probes capable of specific quantification of antigens directly in clinical samples. A nanoconjugation strategy that allows one to perform an intensity depletion immuno-linked assay (IDILA), involving specific antibodies that target the antigen of interest was designed to obtain a calibration curve and achieve the quantification of the antigen in clinical samples in the same experiment using a microplate reader (i.e., an UV-vis spectrophotometer). Finally, the IDILA methodology allowed specific detection of various clinically relevant antigens, with significantly improved sensitivity over the ELISA. Furthermore, the assay was shown to be robust, reliable, cheap and rapid, diagnosing antigens in clinical serum samples within 2 hours.
迄今为止，虽然已经提出了各种用于抗原检测的诊断方法，但大多数方法过于昂贵、耗时且灵敏度有限，不适合临床使用。然而，具有独特材料特性的纳米粒子系统规避了这些问题，提供了比酶联免疫吸附测定（ELISA）等当前方法更高的准确性和灵敏度。在此，我们提出了一种新的等离子体纳米粒子探针功能化策略，能够直接在临床样本中对抗原进行特异性定量。设计了一种纳米缀合策略，允许进行强度耗散免疫连接测定（IDILA），涉及针对感兴趣抗原的特异性抗体，以获得校准曲线并使用微孔板读数器（即，紫外可见分光光度计）在同一实验中实现临床样本中抗原的定量。最后，IDILA 方法允许对各种临床相关抗原进行特异性检测，其灵敏度明显高于 ELISA。此外，该测定方法表现出稳健、可靠、廉价和快速的特点，可在 2 小时内诊断临床血清样本中的抗原。
