Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, USA.
Nat Nanotechnol. 2013 May;8(5):369-75. doi: 10.1038/nnano.2013.70. Epub 2013 May 5.
So far, although various diagnostic approaches for pathogen detection have been proposed, most are too expensive, lengthy or limited in specificity for clinical use. Nanoparticle systems with unique material properties, however, circumvent these problems and offer improved accuracy over current methods. Here, we present novel magneto-DNA probes capable of rapid and specific profiling of pathogens directly in clinical samples. A nanoparticle hybridization assay, involving ubiquitous and specific probes that target bacterial 16S rRNAs, was designed to detect amplified target DNAs using a miniaturized NMR device. Ultimately, the magneto-DNA platform will allow both universal and specific detection of various clinically relevant bacterial species, with sensitivity down to single bacteria. Furthermore, the assay is robust and rapid, simultaneously diagnosing a panel of 13 bacterial species in clinical specimens within 2 h. The generic platform described could be used to rapidly identify and phenotype pathogens for a variety of applications.
到目前为止，尽管已经提出了各种用于病原体检测的诊断方法，但大多数方法都过于昂贵、耗时或特异性有限，不适合临床使用。然而，具有独特材料特性的纳米粒子系统克服了这些问题，并提供了比当前方法更高的准确性。在这里，我们提出了一种新型的磁 DNA 探针，它能够直接在临床样本中快速、特异性地分析病原体。设计了一种涉及普遍存在和特异性探针的纳米粒子杂交测定法，这些探针针对细菌 16S rRNAs，用于使用小型化 NMR 设备检测扩增的靶 DNA。最终，该磁 DNA 平台将允许对各种具有临床相关性的细菌物种进行通用和特异性检测，灵敏度可达单个细菌。此外，该测定法具有稳健和快速的特点，能够在 2 小时内同时诊断临床标本中的 13 种细菌。所描述的通用平台可用于快速识别和表型分析各种病原体，适用于多种应用。
