Smith A, Momany M
University of Georgia, Athens, GA, United States.
Methods Cell Biol. 2016;136:135-41. doi: 10.1016/bs.mcb.2016.03.027. Epub 2016 Apr 20.
Individual septins from separate phylogenetic groups assemble into heteropolymer rods with defined compositions. Heteropolymer rods can in turn assemble into larger higher-order structures (HOS). Using a combination of immunoprecipitation and fluorescence microscopy in Aspergillus nidulans wild-type and septin-mutant strains, we have shown that even when septin HOS are not visible by fluorescence microscopy, heteropolymer rods can still be present and that a septin found largely in filamentous fungi is required for a subset of HOS. We raise several factors that are important for analysis of heteropolymer rods and HOS.
来自不同系统发育组的单个septin蛋白组装成具有特定组成的异源聚合物棒。异源聚合物棒进而可以组装成更大的高阶结构（HOS）。通过在构巢曲霉野生型和septin突变体菌株中结合使用免疫沉淀和荧光显微镜技术，我们已经表明，即使荧光显微镜无法观察到septin高阶结构，异源聚合物棒仍然可能存在，并且丝状真菌中大量存在的一种septin蛋白是一部分高阶结构所必需的。我们提出了几个对异源聚合物棒和高阶结构分析很重要的因素。
