Laboratorio di Spettrometria di Massa Analitica ed Isotopica, Dipartimento di Beni Culturali, Università del Salento, Lecce, Italy.
Laboratorio di Spettrometria di Massa Analitica ed Isotopica, Dipartimento di Beni Culturali, Università del Salento, Lecce, Italy.
Talanta. 2016 Oct 1;159:40-46. doi: 10.1016/j.talanta.2016.06.004. Epub 2016 Jun 3.
This work presents an analytical procedure based on gas chromatography-mass spectrometry which allows the determination of aldoses (glucose, mannose, galactose, arabinose, xylose, fucose, rhamnose) and chetoses (fructose) in plant material. One peak for each target carbohydrate was obtained by using an efficient derivatization employing methylboronic acid and acetic anhydride sequentially, whereas the baseline separation of the analytes was accomplished using an ionic liquid capillary column. First, the proposed method was optimized and validated. Successively, it was applied to identify the carbohydrates present in plant material. Finally, the procedure was successfully applied to samples from a XVII century painting, thus highlighting the occurrence of starch glue and fruit tree gum as polysaccharide materials.
本工作提出了一种基于气相色谱-质谱联用的分析方法，可用于测定植物材料中的醛糖（葡萄糖、甘露糖、半乳糖、阿拉伯糖、木糖、岩藻糖、鼠李糖）和己糖（果糖）。通过使用甲基硼酸和乙酸酐依次进行有效的衍生化，可获得每个目标碳水化合物的一个峰，而使用离子液体毛细管柱则可实现分析物的基线分离。首先，对所提出的方法进行了优化和验证。随后，将其应用于鉴定植物材料中的碳水化合物。最后，该方法成功应用于 17 世纪绘画的样品中，从而凸显了淀粉胶和果树胶作为多糖材料的存在。
