LAFFEX-Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Parnaíba, 64202-020 PI, Brazil.
Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, 60455-760 CE, Brazil; Federal Institute of Education, Science and Technology of Ceará, Juazeiro do Norte, CE, Brazil.
Carbohydr Polym. 2016 Oct 20;151:957-964. doi: 10.1016/j.carbpol.2016.06.047. Epub 2016 Jun 14.
Sulfated polysaccharides extracted from seaweed have important pharmacological properties. Thus, the aim of this study was to characterize the sulfated polysaccharide (PLS) from the algae Hypnea musciformis and evaluate its protective effect in colitis induced by trinitrobenzene sulfonic acid in rats. The sulfated polysaccharide possess a high molecular mass (1.24×10(5)gmol(-1)) and is composed of a κ-carrageenan, as depicted by FT-IR and NMR spectroscopic data. PLS was administered orally (10, 30, and 60mg/kg, p.o.) for three days, starting before TNBS (trinitrobenzene sulfonic acid) instillation (day 1). The rats were killed on day three, the portion of distal colon (5cm) was excised and evaluated macroscopic scores and wet weight. Then, samples of the intestinal were used for histological evaluation and quantification of glutathione, malonyldialdehyde acid, myeloperoxidase, nitrate/nitrite and cytokines. Our results demonstrate that PLS reduced the colitis and all analyzed biochemical parameters. Thus, we concluded that the PLS extracted from the marine algae H. musciformis reduced the colitis in animal model and may have an important promising application in the inflammatory bowel diseases.
从海藻中提取的硫酸多糖具有重要的药理学特性。因此，本研究的目的是对来自藻类 Hypnea musciformis 的硫酸多糖（PLS）进行表征，并评估其在大鼠三硝基苯磺酸诱导结肠炎中的保护作用。硫酸多糖具有高分子质量（1.24×10(5)gmol(-1))，由 FT-IR 和 NMR 光谱数据表明其由 κ-卡拉胶组成。PLS 经口（10、30 和 60mg/kg，po）给药，在 TNBS（三硝基苯磺酸）灌注（第 1 天）前开始连续给药 3 天。大鼠于第 3 天处死，切除远端结肠（5cm）部分并评估宏观评分和湿重。然后，使用肠道样本进行组织学评估和谷胱甘肽、丙二醛酸、髓过氧化物酶、硝酸盐/亚硝酸盐和细胞因子的定量。我们的结果表明，PLS 减轻了结肠炎和所有分析的生化参数。因此，我们得出结论，从海洋藻类 H. musciformis 中提取的 PLS 减轻了动物模型中的结肠炎，并且在炎症性肠病中可能具有重要的应用前景。
