Li J, Yang H, Meng S, Zhou J, Ding Y
Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China; Department of Implantology, the Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, PR China.
Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China; Department of Periodontology, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China.
J Ethnopharmacol. 2016 Dec 4;193:248-254. doi: 10.1016/j.jep.2016.07.051. Epub 2016 Jul 27.
ETHNO-PHARMACOLOGICAL RELEVANCE: Bu-Shen-Gu-Chi-Wan is a Chinese patent medicine for the treatment of chronic periodontitis. It has an anti-inflammatory function in experimental periodontitis and can improve alveolar bone remodeling.
This study aims to explore the effects of Bu-Shen-Gu-Chi-Wan on serum metabolism in a rat model of periodontitis using H nuclear magnetic resonance (H NMR) based metabonomics.
The model of experimental periodontitis in a rat was established by steel wire ligation, plus a high glucose diet and Porphyromonas gingivalis inoculation. When the models had been established, 6-week-old Sprague-Dawley female rats (n=31) were divided into 5 groups: high dose group (Group H), medium dose group (Group M), low dose group (Group L), periodontitis group (Group P) and healthy control group (Group N). Rats in Group H, M and L were given the Bu-Shen-Gu-Chi-Wan solution (0.8, 2 and 4g/kg of body weight) daily for 60 days. Rats in Group P and N were administered normal saline (10ml/kg of body weight) in the same period. All rats were sacrificed at the end of the study and serum samples were collected. The metabolites in the serum were analyzed using H NMR in conjunction with principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA).
The metabonomic profiles of five groups showed the clustering of individual dataset in every group, and the 1st principal component and the 2nd principal component could significantly differentiate the datasets of medium dose or high dose group from other groups. The chemical shift regions at δ 1.22ppm, 1.86ppm, 2.26ppm, 2.34ppm and 2.42ppm showed the most obvious differences among the five groups. The correspondent metabolites were high density lipoprotein, pyruvate/oxaloacetate, arginine and glutamine.
The effects of Bu-Shen-Gu-Chi-Wan on the rat serum metabolites were dose dependent. High density lipoprotein, pyruvate/oxaloacetate, arginine and glutamine may be the serum biomarkers of Bu-Shen-Gu-Chi-Wan.
补肾固齿丸是一种用于治疗慢性牙周炎的中成药。它在实验性牙周炎中具有抗炎作用，并能改善牙槽骨重塑。
本研究旨在利用基于氢核磁共振（H NMR）的代谢组学方法，探讨补肾固齿丸对牙周炎大鼠模型血清代谢的影响。
通过钢丝结扎、高糖饮食和接种牙龈卟啉单胞菌建立大鼠实验性牙周炎模型。模型建立后，将6周龄的雌性Sprague-Dawley大鼠（n = 31）分为5组：高剂量组（H组）、中剂量组（M组）、低剂量组（L组）、牙周炎组（P组）和健康对照组（N组）。H组、M组和L组大鼠每天给予补肾固齿丸溶液（0.8、2和4 g/kg体重），持续60天。P组和N组大鼠在同一时期给予生理盐水（10 ml/kg体重）。研究结束时处死所有大鼠并收集血清样本。采用H NMR结合主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）对血清中的代谢物进行分析。
五组的代谢组学图谱显示每组个体数据集的聚类，第一主成分和第二主成分能够显著区分中剂量或高剂量组与其他组的数据集。δ 1.22ppm、1.86ppm、2.26ppm、2.34ppm和2.42ppm处的化学位移区域在五组之间显示出最明显的差异。相应的代谢物为高密度脂蛋白、丙酮酸/草酰乙酸、精氨酸和谷氨酰胺。
补肾固齿丸对大鼠血清代谢物的影响呈剂量依赖性。高密度脂蛋白、丙酮酸/草酰乙酸、精氨酸和谷氨酰胺可能是补肾固齿丸的血清生物标志物。
