Yang H, Wen Q, Xue J, Ding Y
Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Sichuan, China; Department of Periodontology, West China Hospital of Stomatology, Sichuan University, Sichuan, China.
J Periodontal Res. 2014 Jun;49(3):382-9. doi: 10.1111/jre.12117. Epub 2013 Aug 27.
The aim of this study was to investigate the effect of a traditional Chinese medicine, Bu-Shen-Gu-Chi-Wan, on experimental periodontitis and bone regeneration in rat.
Sixty female Sprague-Dawley rats were divided equally into three groups: a healthy control group (Group N); a periodontitis group (Group P); and the Bu-Shen-Gu-Chi-Wan treatment group (Group T). A 0.2-mm wire was placed around the maxillary first molar and Porphyromonas gingivalis was injected into the gingival sulcus. Rats in different groups were administered 0.9% normal saline or Bu-Shen-Gu-Chi-Wan solution (4 g/kg of body weight, for three alternate days), and the animals were killed after 4 wk. Morphological analysis of alveolar bone rebuilding was performed using micro-computed tomography (micro-CT) and stereomicroscopy, and the variation of inflammation in the periodontium was determined histologically. The serum levels of the proinflammatory cytokines interleukin-1beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) and of the bone-turnover biomarkers pyridinoline cross-linked carboxyterminal telopeptide of type I collagen (ICTP) and osteocalcin (OC) were determined using radioimmunoassays.
After treatment with Bu-Shen-Gu-Chi-Wan, there were significant decreases in the levels of IL-1β, TNF-α, ICTP and OC and decreased inflammatory infiltration in the periodontal tissues of Group T. significant changes in alveolar bone volume and density were detected by micro-CT, but stereomicroscopy did not detect a significant improvement of alveolar bone height.
The data of the present study suggest that the traditional Chinese medicine, Bu-Shen-Gu-Chi-Wan, has anti-inflammatory function in experimental periodontitis and may simultaneously improve alveolar bone remodeling.
本研究旨在探讨中药补肾固齿丸对大鼠实验性牙周炎及骨再生的影响。
60只雌性Sprague-Dawley大鼠平均分为三组：健康对照组（N组）；牙周炎组（P组）；补肾固齿丸治疗组（T组）。在上颌第一磨牙周围放置0.2毫米的丝线，并将牙龈卟啉单胞菌注入牙龈沟。不同组的大鼠给予0.9%生理盐水或补肾固齿丸溶液（4克/千克体重，隔日给药一次），4周后处死动物。使用微型计算机断层扫描（micro-CT）和体视显微镜对牙槽骨重建进行形态学分析，并通过组织学方法确定牙周组织炎症的变化。采用放射免疫分析法测定促炎细胞因子白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）以及骨转换生物标志物I型胶原吡啶交联羧基末端肽（ICTP）和骨钙素（OC）的血清水平。
补肾固齿丸治疗后，T组IL-1β、TNF-α、ICTP和OC水平显著降低，牙周组织炎症浸润减少。micro-CT检测到牙槽骨体积和密度有显著变化，但体视显微镜未检测到牙槽骨高度有显著改善。
本研究数据表明，中药补肾固齿丸在实验性牙周炎中具有抗炎作用，可能同时改善牙槽骨重塑。
