Xu Wenteng, Li Hailong, Dong Zhongdian, Cui Zhongkai, Zhang Ning, Meng Liang, Zhu Ying, Liu Yang, Li Yangzhen, Guo Hua, Ma Jialu, Wei Zhanfei, Zhang Nianwei, Yang Yingming, Chen Songlin
Yellow Sea Fisheries Research Institute, CAFS, Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Yellow Sea Fisheries Research Institute, CAFS, Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Gene. 2016 Oct 30;592(1):215-220. doi: 10.1016/j.gene.2016.07.062. Epub 2016 Jul 30.
E3 ubiquitin ligases are a large gene family that plays a diversity of roles in spermatogenesis. In this study, the functional characterization of a neuralized E3 ubiquitin protein ligase 3 (neurl3) revealed its potential participation in spermatogenesis. Firstly, we found that neurl3 exhibited male-biased transcription and that its translation was predominant in testis germ cells. The knockdown of neurl3 by RNA interference caused increased transcription of spermatogenesis-related genes. These results corroborate previous studies indicating a role for neurl3 in spermatogenesis. Moreover, the levels of neurl3 transcription and testis protein ubiquitination were closely correlated. Based on these findings, we speculate that neurl3 modulates testis protein ubiquitination in a dosage-dependent manner and that this influences spermatogenesis.
E3泛素连接酶是一个庞大的基因家族，在精子发生过程中发挥着多种作用。在本研究中，对神经化E3泛素蛋白连接酶3（neurl3）的功能表征揭示了其在精子发生中的潜在作用。首先，我们发现neurl3表现出雄性偏向性转录，并且其翻译在睾丸生殖细胞中占主导地位。通过RNA干扰敲低neurl3导致精子发生相关基因的转录增加。这些结果证实了先前的研究，表明neurl3在精子发生中发挥作用。此外，neurl3转录水平与睾丸蛋白泛素化水平密切相关。基于这些发现，我们推测neurl3以剂量依赖的方式调节睾丸蛋白泛素化，进而影响精子发生。
