Uyy Elena, Suica Viorel I, Boteanu Raluca M, Manda Dana, Baciu Ancuta E, Badiu Corin, Antohe Felicia
Institute of Cellular Biology and Pathology "Nicolae Simionescu" , 8, B.P. Hasdeu Street, P.O. Box 35-14, 050568 Bucharest, Romania.
National Institute of Endocrinology "C.I. Parhon" , 34-36 Aviatorilor Boulevard, 011863 Bucharest, Romania.
J Proteome Res. 2016 Sep 2;15(9):3377-87. doi: 10.1021/acs.jproteome.6b00567. Epub 2016 Aug 16.
The study aimed to evaluate the proteomic changes in benign follicular adenoma versus malignant follicular variant of papillary thyroid carcinoma. Tumor and nontumor adjacent samples were analyzed by liquid nanochromatography mass spectrometry, and protein abundance was evaluated by label-free quantification. Western blotting and quantitative real-time polymerase chain reaction were used to validate and complement the mass spectrometry data. The results demonstrated deregulated expression of four endoplasmic reticulum chaperones (78 kDa glucose-regulated protein, endoplasmin, calnexin, protein disulfide-isomerase A4), glutathione peroxidase 3 and thyroglobulin, all of them involved in thyroid hormone synthesis pathway. The altered tissue abundance of endoplasmic reticulum chaperones in thyroid cancer was correlated with serum expression levels. The identified proteins significantly discriminate between adenoma and carcinoma in both thyroid tissue and corresponding sera. Data are available via ProteomeXchange with identifier PXD004322.
该研究旨在评估良性滤泡性腺瘤与甲状腺乳头状癌滤泡状变异型恶性肿瘤之间的蛋白质组学变化。通过液相纳米色谱质谱分析法对肿瘤及肿瘤旁非肿瘤样本进行分析，并采用无标记定量法评估蛋白质丰度。利用蛋白质免疫印迹法和定量实时聚合酶链反应对质谱数据进行验证和补充。结果表明，四种内质网伴侣蛋白（78 kDa葡萄糖调节蛋白、内质蛋白、钙连接蛋白、蛋白二硫键异构酶A4）、谷胱甘肽过氧化物酶3和甲状腺球蛋白的表达失调，它们均参与甲状腺激素合成途径。甲状腺癌中内质网伴侣蛋白组织丰度的改变与血清表达水平相关。所鉴定的蛋白质在甲状腺组织和相应血清中均能显著区分腺瘤和癌。数据可通过ProteomeXchange获得，标识符为PXD004322。
