Institute of Cellular Biology and Pathology "N. Simionescu" of the Romanian Academy, 8, B.P. Hasdeu Street, PO Box 35-14, 050568, Bucharest, Romania.
Faculty of Engineering and Management of Technological Systems, Politehnica University of Bucharest, Bucharest, Romania.
Sci Rep. 2020 Oct 28;10(1):18493. doi: 10.1038/s41598-020-75527-2.
Due to their excellent mechanical and biocompatibility properties, titanium-based implants are successfully used as biomedical devices. However, when new bone formation fails for different reasons, impaired fracture healing becomes a clinical problem and affects the patient's quality of life. We aimed to design a new bioactive surface of titanium implants with a synergetic PEG biopolymer-based composition for gradual delivery of growth factors (FGF2, VEGF, and BMP4) during bone healing. The optimal architecture of non-cytotoxic polymeric coatings deposited by dip coating under controlled parameters was assessed both in cultured cells and in a rat tibial defect model (100% viability). Notably, the titanium adsorbed polymer matrix induced an improved healing process when compared with the individual action of each biomolecules. High-performance mass spectrometry analysis demonstrated that recovery after a traumatic event is governed by specific differentially regulated proteins, acting in a coordinated response to the external stimulus. Predicted protein interactions shown by STRING analysis were well organized in hub-based networks related with response to chemical, wound healing and response to stress pathways. The proposed functional polymer coatings of the titanium implants demonstrated the significant improvement of bone healing process after injury.
由于其出色的机械和生物相容性，基于钛的植入物被成功地用作生物医学设备。然而，当由于各种原因导致新骨形成失败时，受损的骨折愈合就成为一个临床问题，并影响患者的生活质量。我们旨在设计一种新的钛植入物的生物活性表面，其具有协同的基于聚乙二醇（PEG）生物聚合物的组成，用于在骨愈合过程中逐渐递呈生长因子（FGF2、VEGF 和 BMP4）。通过控制参数的浸渍涂层沉积的非细胞毒性聚合物涂层的最佳结构，在培养细胞和大鼠胫骨缺损模型中进行了评估（100%存活率）。值得注意的是，与每种生物分子的单独作用相比，吸附在钛上的聚合物基质诱导了改善的愈合过程。高性能质谱分析表明，创伤后的恢复受特定差异调节蛋白的控制，这些蛋白以协调的方式对外部刺激做出反应。STRING 分析显示的预测蛋白相互作用很好地组织在与化学、伤口愈合和应激反应途径相关的基于枢纽的网络中。所提出的钛植入物的功能聚合物涂层显示出在受伤后显著改善了骨愈合过程。
