Hayashi Miri, Maehara Seiya, Ito Yosuke, Yamashita Kazuto, Kubo Akira, Nakade Tetsuya
Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Vet Ophthalmol. 2017 Jul;20(4):310-315. doi: 10.1111/vop.12418. Epub 2016 Aug 2.
To study a new approach to indocyanine green (ICG) angiography for contrasting the ocular fundus in cats.
Six healthy laboratory cats.
Fluorescein (FLUO) and ICG angiography were performed using an infrared-sensitive charged coupled device-equipped fundus camera on sedated cats.
At 12.3 ± 3.4 s after ICG administration, the choroidal arteries could be seen extending radially from the optic disk. The choroidal veins became apparent at 16.2 ± 4.1 s alongside the choroidal arteries. Gradual fading of the choroidal vessels began 5.8 ± 1.5 min postdye administration and diffuse fluorescence of the fundus appeared. Diffuse fluorescence of the optic disk faded at about 18.8 ± 2.9 min. Mean arterial blood pressure at 1 and 3 min after ICG administration showed no significant change when compared to pre-administration (P > 0.05). However, 5 min (P = 0.054) and 10 min (P < 0.05) postadministration, a significant drop in blood pressure occurred. The time lapse between FLUO administration and its appearance in the ocular fundus was 15.7 ± 3.8 s. Retinal veins became apparent at 22.0 ± 3.6 s alongside retinal arteries. At 31.2 ± 4.1 s, full venous fluorescence was visualized throughout the entire fundus.
While FLUO angiography shows only the retinal vessels, ICG angiography enabled visualization of the choroidal vasculature. ICG angiography provides clear resolution while remaining reliable and simple; thus, a combination of ICG and FLUO angiography shows promise as a diagnostic aid for clinical evaluation of various chorioretinal diseases in cats.
研究一种用于猫眼底造影的新型吲哚菁绿（ICG）血管造影方法。
6只健康的实验猫。
在镇静的猫身上，使用配备红外敏感电荷耦合器件的眼底相机进行荧光素（FLUO）和ICG血管造影。
注射ICG后12.3±3.4秒，可见脉络膜动脉从视盘呈放射状延伸。脉络膜静脉在16.2±4.1秒时与脉络膜动脉一同显现。染料注射后5.8±1.5分钟，脉络膜血管开始逐渐消退，眼底出现弥漫性荧光。视盘的弥漫性荧光在约18.8±2.9分钟时消退。注射ICG后1分钟和3分钟时的平均动脉血压与注射前相比无显著变化（P>0.05）。然而，注射后5分钟（P = 0.054）和10分钟（P<0.05）时，血压出现显著下降。注射FLUO到其在眼底出现的时间间隔为15.7±3.8秒。视网膜静脉在22.0±3.6秒时与视网膜动脉一同显现。在31.2±4.1秒时，整个眼底可见完全的静脉荧光。
虽然FLUO血管造影仅显示视网膜血管，但ICG血管造影能够使脉络膜血管系统可视化。ICG血管造影提供了清晰的分辨率，同时保持可靠且简单；因此，ICG和FLUO血管造影相结合有望作为猫各种脉络膜视网膜疾病临床评估的诊断辅助手段。
