采用集落形成试验测定贴壁细胞的存活率。

Measuring Survival of Adherent Cells with the Colony-Forming Assay.

作者信息

Crowley Lisa C, Christensen Melinda E, Waterhouse Nigel J

机构信息

Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia;

Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia; Flow Cytometry and Imaging, QIMR Berghofer Medical Research Institute, Herston, Brisbane, Queensland 4006, Australia; Division of Immunology, Mater Pathology, Mater Adult Hospital, South Brisbane, Queensland 4101, Australia;

出版信息

Cold Spring Harb Protoc. 2016 Aug 1;2016(8):2016/8/pdb.prot087171. doi: 10.1101/pdb.prot087171.

DOI:10.1101/pdb.prot087171

PMID:27480717

Abstract

Measuring cell death with colorimetric or fluorimetric dyes such as trypan blue and propidium iodide (PI) can provide an accurate measure of the number of dead cells in a population at a specific time; however, these assays cannot be used to distinguish cells that are dying or marked for future death. In many cases it is essential to measure the proliferative capacity of treated cells to provide an indirect measurement of cell death. This can be achieved using the colony-forming assay described here. This protocol specifically applies to measurement of HeLa cells but can be used for most adherent cell lines with limited motility.

摘要

使用比色或荧光染料（如台盼蓝和碘化丙啶（PI））来测量细胞死亡，可以准确测量特定时间群体中死细胞的数量；然而，这些检测方法无法用于区分正在死亡或标记为未来死亡的细胞。在许多情况下，测量处理后细胞的增殖能力以间接测量细胞死亡至关重要。这可以通过此处描述的集落形成试验来实现。该方案特别适用于HeLa细胞的测量，但也可用于大多数运动能力有限的贴壁细胞系。