猪微生物群中大肠杆菌携带多重耐药基因的IncI/ST12质粒的罕见自发丢失
Rare Spontaneous Loss of Multiresistance Gene Carrying IncI/ST12 Plasmid in Escherichia coli in Pig Microbiota.
作者信息
Mourand Gwenaelle, Touzain Fabrice, Jouy Eric, Fleury Mickael Alain, Denamur Erick, Kempf Isabelle
机构信息
Laboratoire de Ploufragan-Plouzané, Agence Nationale de Sécurité Sanitaire (Anses), Université Bretagne Loire, Ploufragan, France.
INSERM, IAME, UMR1137, Paris, France Université Paris Diderot, IAME, UMR1137, Sorbonne Paris Cité, Paris, France AP-HP, Laboratoire de Génétique Moléculaire, GH Paris Nord Val de Seine, Paris, France.
出版信息
Antimicrob Agents Chemother. 2016 Sep 23;60(10):6046-9. doi: 10.1128/AAC.00864-16. Print 2016 Oct.
Resistance to extended-spectrum cephalosporins (ESCs) is a matter of considerable concern for public health. Here, we studied the spontaneous loss of an extended-spectrum beta-lactamase (ESBL)-encoding plasmid from a rifampin-resistant Escherichia coli isolate orally inoculated into pigs under controlled conditions. Fecal samples were collected and cultured on rifampin-supplemented medium, and the resistance of the E. coli isolates to ESCs was studied by phenotypic tests, PCR detection of plasmid genes, and complete sequencing. The results showed that only 3 out of 353 rifampin-resistant E. coli isolates were ESC susceptible, and PCR and bioinformatics analysis confirmed the loss of the plasmid. These in vivo experiments indicate that the loss of an ESBL-encoding plasmid seems a rare event in gut microbiota.
对超广谱头孢菌素（ESCs）的耐药性是公共卫生领域相当关注的问题。在此，我们研究了在可控条件下经口接种到猪体内的一株耐利福平大肠杆菌分离株中编码超广谱β-内酰胺酶（ESBL）的质粒的自发丢失情况。收集粪便样本并在添加利福平的培养基上进行培养，通过表型试验、质粒基因的PCR检测和全序列测定研究大肠杆菌分离株对ESCs的耐药性。结果显示，353株耐利福平大肠杆菌分离株中只有3株对ESCs敏感，PCR和生物信息学分析证实了质粒的丢失。这些体内实验表明，编码ESBL的质粒丢失在肠道微生物群中似乎是一个罕见事件。
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