Zhang Junyan, Huang Suzhen, Ma Yuanyuan, Zhang Minhua, Zou Shaolan
R&D Center for Petrochemical Technology, Tianjin University, Tianjin, 300072, China.
Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
Biotechnol Lett. 2016 Nov;38(11):1929-1934. doi: 10.1007/s10529-016-2180-6. Epub 2016 Aug 1.
A new solvent-tolerant species, Staphylococcus aureus, was isolated and characterized during the screening of butanol-tolerant microorganisms.
Three isolates of S. aureus were obtained as contaminants during improvement of butanol tolerance of E. coli K12. Their cell dry weights were 135 % that of K12 in the absence of butanol stress. S. aureus had a growth advantage over K12 when cultured with various concentrations of butanol. It can tolerate up to 3 % (v/v) butanol, while most solventogenic bacteria can tolerate only 2 % (v/v) butanol. The addition of 10-20 g glucose/l enhanced its butanol tolerance. The relative cell biomass of the S. aureus was 71-306 % that of E. coli under 5.5-10 % (v/v) ethanol stress, indicating ethanol resistance.
This is the first study to observe butanol-tolerant S. aureus. As this organism can be genetically manipulated, it could have a wide array of applications.
在筛选耐丁醇微生物的过程中，分离并鉴定了一种新的耐溶剂金黄色葡萄球菌。
在提高大肠杆菌K12耐丁醇能力的过程中，获得了三株金黄色葡萄球菌作为污染物。在无丁醇胁迫的情况下，它们的细胞干重是K12的135%。当用不同浓度的丁醇培养时，金黄色葡萄球菌比K12具有生长优势。它能耐受高达3%（v/v）的丁醇，而大多数产溶剂细菌只能耐受2%（v/v）的丁醇。添加10 - 20 g葡萄糖/升可增强其耐丁醇能力。在5.5 - 10%（v/v）乙醇胁迫下，金黄色葡萄球菌的相对细胞生物量是大肠杆菌的71 - 306%，表明其具有乙醇抗性。
这是首次观察到耐丁醇的金黄色葡萄球菌的研究。由于这种生物体可以进行基因操作，它可能有广泛的应用。
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