Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Lett Appl Microbiol. 2010 Apr;50(4):373-9. doi: 10.1111/j.1472-765X.2010.02808.x. Epub 2010 Jan 22.
Poor butanol tolerance of solventogenic stains directly limits their butanol production during industrial-scale fermentation process. This study was performed to search for micro-organisms possessing elevated tolerance to butanol.
Two strains, which displayed higher butanol tolerance compared to commonly used solventogenic Clostridium acetobutylicum, were isolated by evolution and screening strategies. Both strains were identified as lactic acid bacteria (LAB). On this basis, a LAB culture collection was tested for butanol tolerance, and 60% of the strains could grow at a butanol concentration of 2.5% (v/v). In addition, an isolated strain with superior butanol tolerance was transformed using a certain plasmid.
The results indicate that many strains of LAB possessed inherent tolerance of butanol.
This study suggests that LAB strains may be capable of producing butanol to elevated levels following suitable genetic manipulation.
溶剂梭菌菌株对丁醇的耐受性差，直接限制了它们在工业规模发酵过程中的丁醇产量。本研究旨在寻找对丁醇具有更高耐受性的微生物。
通过进化和筛选策略，分离到两株与常用溶剂梭菌 Clostridium acetobutylicum 相比具有更高丁醇耐受性的菌株。这两株菌均被鉴定为乳酸菌（LAB）。在此基础上，对乳酸菌培养物进行了丁醇耐受性测试，其中 60%的菌株可以在 2.5%（v/v）的丁醇浓度下生长。此外，通过某种质粒对具有较高丁醇耐受性的分离菌株进行了转化。
结果表明，许多乳酸菌菌株具有内在的丁醇耐受性。
本研究表明，经过适当的遗传操作，LAB 菌株可能能够生产出更高水平的丁醇。
