伊朗西阿塞拜疆省绵羊和牛中绵羊无形体和边缘无形体感染率的分子研究
Molecular study on infection rates of Anaplasma ovis and Anaplasma marginale in sheep and cattle in West-Azerbaijan province, Iran.
作者信息
Noaman Vahid, Bastani Davood
机构信息
Veterinary Research Department, Isfahan Agriculture and Natural Resources Research and Education Center, AREEO , Isfahan, Iran;
Graduate Student, Faculty of Veterinary Medicine, Islamic Azad University, Boroujerd Branch, Boroujerd, Iran.
出版信息
Vet Res Forum. 2016 Spring;7(2):163-7. Epub 2016 Jun 15.
This study was carried out to determine the presence and frequency of Anaplasma ovis and Anaplasma marginale in sheep and dairy cattle in West-Azerbaijan province, Iran. A total number of 200 blood samples were randomly collected via the jugular vein from apparently healthy cattle (100) and sheep (100). The extracted DNA from blood cells was screened using genus-specific (Anaplasma spp.) nested-polymerase chain reaction (PCR) based on 16S rRNA gene primer sets. Species-specific PCR was set up using major surface protein 4 (MSP4) gene primer set. None of cattle blood samples were positive for Anaplasma spp. by the first nested PCR. Five samples among the 100 sheep blood samples were both positive in the first nested PCR and A. ovis -specific PCR, based on MSP4 gene. In total, 5.00% of animals were A. ovis positive. This study identified a low prevalence of A. ovis in the blood of apparently healthy sheep in West Azerbaijan province.
本研究旨在确定伊朗西阿塞拜疆省绵羊和奶牛中绵羊无形体和边缘无形体的存在情况及感染频率。通过颈静脉从100头看似健康的奶牛和100只看似健康的绵羊中随机采集了总共200份血样。使用基于16S rRNA基因引物对的属特异性（无形体属）巢式聚合酶链反应（PCR）对从血细胞中提取的DNA进行筛查。使用主要表面蛋白4（MSP4）基因引物对进行种特异性PCR。在第一次巢式PCR中，没有一份奶牛血样对无形体属呈阳性。在100份绵羊血样中，有5份在第一次巢式PCR和基于MSP4基因的绵羊无形体特异性PCR中均呈阳性。总体而言，5.00%的动物感染了绵羊无形体。本研究发现伊朗西阿塞拜疆省看似健康的绵羊血液中绵羊无形体的感染率较低。
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