de la Fuente José, Torina Alessandra, Caracappa Santo, Tumino Giovanni, Furlá Roberto, Almazán Consuelo, Kocan Katherine M
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElory Hall, Stillwater, OK 74078, USA.
Vet Parasitol. 2005 Nov 5;133(4):357-62. doi: 10.1016/j.vetpar.2005.05.063.
Although Anaplasma marginale was known to be endemic in Italy, the diversity of Anaplasma spp. from this area have not been characterized. In this study, the prevalence of Anaplasma spp. antibodies in randomly selected farm animals collected on the island of Sicily was determined by use of a MSP5 cELISA for Anaplasma spp. and an immunofluorescence test specific for Anaplasma phagocytophilum. Genetic variation among strains of Anaplasma spp. from animals and ticks was characterized using the A. marginale msp1alpha and the Anaplasma spp. msp4 genes. Eight species of ticks were collected and tested by PCR. Seropositivity for Anaplasma spp. and A. phagocytophilum was detected in bovine and ovine samples. All the donkeys were seropositive for A. phagocytophilum but not for Anaplasma spp. Four A. marginale genotypes were identified by msp4 sequences from bovine and tick samples. Two new genotypes of Anaplasma ovis were characterized in sheep. The sequences of A. phagocytophilum from three donkeys proved to be identical to the sequence of the MRK equine isolate from California. Six A. marginale genotypes were found in cattle and one tick using the A. marginale msp1alpha sequences. All genotypes had four repeated sequences in the N-terminal portion of the MSP1a, except for one that had five repeats. The Italian strains of A. marginale contained three repeat sequences that were not reported previously. Definition of the diversity of Anaplasma spp. in Sicily reported, herein is fundamental to development of control strategies for A. marginale, A. ovis and A. phagocytophilum in Sicily.
虽然已知边缘无形体在意大利为地方病，但该地区无形体属的多样性尚未得到描述。在本研究中，通过使用针对无形体属的MSP5 cELISA和针对嗜吞噬细胞无形体的免疫荧光试验，测定了在西西里岛随机采集的农场动物中无形体属抗体的流行率。利用边缘无形体msp1alpha和无形体属msp4基因对来自动物和蜱的无形体属菌株之间的遗传变异进行了描述。采集了8种蜱并通过PCR进行检测。在牛和羊的样本中检测到无形体属和嗜吞噬细胞无形体的血清阳性。所有的驴对嗜吞噬细胞无形体血清呈阳性，但对无形体属血清呈阴性。通过牛和蜱样本的msp4序列鉴定出4种边缘无形体基因型。在绵羊中鉴定出两种新的绵羊无形体基因型。来自三头驴的嗜吞噬细胞无形体序列被证明与来自加利福尼亚的MRK马分离株的序列相同。使用边缘无形体msp1alpha序列在牛和一只蜱中发现了6种边缘无形体基因型。除了一种有5个重复序列的基因型外，所有基因型在MSP1a的N端部分都有4个重复序列。意大利的边缘无形体菌株包含三个以前未报道过的重复序列。本文报道的西西里岛无形体属多样性的定义对于西西里岛边缘无形体、绵羊无形体和嗜吞噬细胞无形体控制策略的制定至关重要。
