Tu Yan, Chen Yadong, Zheng Chongyang, Chen Hui
Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, Zhejiang, China.
J Infect Dev Ctries. 2016 Aug 2;10(7):704-11. doi: 10.3855/jidc.6639.
The purpose of this study was to observe platelet aggregation promoted by biofilms of Streptococcus sanguinis and Porphyromonas gingivalis and to evaluate the effect of two different mouth rinses on this process.
In the first experiment, the same amount of S. sanguinis, P. gingivalis, and the S. sanguinis + P. gingivalis mixed solution was added to an equivalent amount of platelet-rich plasma (PRP). Aggregation was measured using a recording platelet aggregometer. In the second experiment, S. sanguinis, P. gingivalis, S sanguinis + P. gingivalis mixed solutions were pretreated with either Listerine antiseptic mouth rinse or Xipayi mouth rinse for 3 minutes, 6 minutes, and 10 minutes, respectively. The same amount of solution was added to the PRP, and the inhibition of aggregation was measured.
In the first experiment, S. sanguinis and P. gingivalis were able to induce platelet aggregation. The aggregation rate of S. sanguinis + P. gingivalis was significantly lower than that of either S. sanguinis or P. gingivalis. In the second experiment, when S. sanguinis, P. gingivalis, and the S. sanguinis + P. gingivalis mixed solutions were pretreated with Listerine antiseptic mouth rinse for 3 minutes and Xipayi mouth rinse for 10 minutes, there was no significant platelet aggregation.
Platelets could adhere to S. sanguinis or P. gingivalis, but when S. sanguinis was mixed with P. gingivalis, the aggregation rate was reduced significantly. Treatment with Listerine antiseptic mouth rinse or Xipayi mouth rinse inhibited the ability of the bacteria to induce platelet aggregation.
本研究的目的是观察血链球菌和牙龈卟啉单胞菌生物膜促进血小板聚集的情况，并评估两种不同漱口水对该过程的影响。
在第一个实验中，将等量的血链球菌、牙龈卟啉单胞菌以及血链球菌+牙龈卟啉单胞菌混合溶液添加到等量的富血小板血浆（PRP）中。使用记录式血小板聚集仪测量聚集情况。在第二个实验中，血链球菌、牙龈卟啉单胞菌、血链球菌+牙龈卟啉单胞菌混合溶液分别用李施德林抗菌漱口水或西帕依固龈液预处理3分钟、6分钟和10分钟。将等量的溶液添加到PRP中，并测量聚集抑制情况。
在第一个实验中，血链球菌和牙龈卟啉单胞菌能够诱导血小板聚集。血链球菌+牙龈卟啉单胞菌的聚集率显著低于血链球菌或牙龈卟啉单胞菌。在第二个实验中，当血链球菌、牙龈卟啉单胞菌以及血链球菌+牙龈卟啉单胞菌混合溶液用李施德林抗菌漱口水预处理3分钟和用西帕依固龈液预处理10分钟时，未出现明显的血小板聚集。
血小板可黏附于血链球菌或牙龈卟啉单胞菌，但当血链球菌与牙龈卟啉单胞菌混合时，聚集率显著降低。用李施德林抗菌漱口水或西帕依固龈液处理可抑制细菌诱导血小板聚集的能力。
