通过口服表达来自重组植物病毒的植物内RNAi效应子来沉默粘虫几丁质酶基因。
Silencing of Mythimna separata chitinase genes via oral delivery of in planta-expressed RNAi effectors from a recombinant plant virus.
作者信息
Bao Wenhua, Cao Budao, Zhang Yanan, Wuriyanghan Hada
机构信息
Inner Mongolia University, No.235 West College Road, Inner Mongolia, Hohhot, P.R.C ZIP: 010021, China.
出版信息
Biotechnol Lett. 2016 Nov;38(11):1961-1966. doi: 10.1007/s10529-016-2186-0. Epub 2016 Aug 2.
OBJECTIVES
To evaluate transient expression of RNA interference (RNAi) effectors in Nicotiana benthamiana plants by using recombinant virus vectors and also oral delivery of the effectors for silencing of Mythimna separata endogenous gene expression.
RESULTS
Mythimna separata is a serious pest of corn production in China. To evaluate RNAi approaches to target specific RNAs in M. separate, we cloned fragments of the M. separata chitinase sequences into a virus vector in order to produce RNAi effectors during virus infection and replication in plants. When the infected plants were fed to M. separata, expression levels of target MseChi1 and MseChi2 genes were down-regulated by 76 and 45 %, respectively, and sequence-specific siRNAs were detected in recipient insects. RNAi-based silencing of chitinase genes also led to body weight decreases by 43 %.
CONCLUSION
Our research demonstrates target mRNA knockdown and suggests a promising application for controlling of M. separata by in planta expression of RNAi effectors using a recombinant plant virus.
目的
利用重组病毒载体评估RNA干扰（RNAi）效应物在本氏烟草植物中的瞬时表达，并通过口服效应物使黏虫内源性基因表达沉默。
结果
黏虫是中国玉米生产中的一种严重害虫。为了评估针对黏虫中特定RNA的RNAi方法，我们将黏虫几丁质酶序列片段克隆到病毒载体中，以便在病毒感染和植物复制过程中产生RNAi效应物。当将受感染的植物喂给黏虫时，目标MseChi1和MseChi2基因的表达水平分别下调了76%和45%，并且在受体昆虫中检测到了序列特异性小干扰RNA（siRNA）。基于RNAi的几丁质酶基因沉默还导致体重下降了43%。
结论
我们的研究证明了靶标mRNA的敲低，并表明利用重组植物病毒在植物中表达RNAi效应物来控制黏虫具有广阔的应用前景。
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