Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland.
Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland.
Bioresour Technol. 2016 Oct;218:1294-7. doi: 10.1016/j.biortech.2016.07.104. Epub 2016 Jul 26.
This study evaluates the effect of different concentrations of glucose supplementation on growth, lipid accumulation, and the fatty acid profile in the Auxenochlorella protothecoides. Addition of glucose promoted the growth rate and decreased the chlorophyll content. Compared with photoautotrophic cells, an increase in the lipid content was observed in mixotrophic cells. The glucose addition induced changes in the fatty acid profile. Higher content of saturated fatty acids was found in the case of cells growing in the glucose-free medium. Oleic acid was the predominant component in mixotrophic cells supplemented with 5gL(-1) glucose, while linoleic acids dominated in cultures supplemented with both 1 and 3gL(-1) glucose. The use of glucose was associated with decreased levels of linolenic acid and PUFA. The changes in the fatty acid profile in mixotrophic cells are favourable for biodiesel production.
本研究评估了不同浓度葡萄糖补加对小球藻生长、脂类积累和脂肪酸组成的影响。葡萄糖的添加促进了生长速率，降低了叶绿素含量。与自养细胞相比，混合营养细胞的脂类含量增加。葡萄糖的添加诱导了脂肪酸组成的变化。在无糖培养基中生长的细胞中发现了更高含量的饱和脂肪酸。在添加 5gL(-1)葡萄糖的混合营养细胞中，油酸是主要成分，而在添加 1 和 3gL(-1)葡萄糖的培养物中，亚油酸占主导地位。葡萄糖的使用与亚麻酸和多不饱和脂肪酸水平的降低有关。混合营养细胞脂肪酸组成的变化有利于生物柴油的生产。
