Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
Bioresour Technol. 2012 Feb;105:120-7. doi: 10.1016/j.biortech.2011.11.103. Epub 2011 Dec 2.
The growth and lipid productivity of an isolated microalga Chlorella vulgaris ESP-31 were investigated under different media and cultivation conditions, including phototrophic growth (NaHCO(3) or CO(2), with light), heterotrophic growth (glucose, without light), photoheterotrophic growth (glucose, with light) and mixotrophic growth (glucose and CO(2), with light). C. vulgaris ESP-31 preferred to grow under phototrophic (CO(2)), photoheterotrophic and mixotrophic conditions on nitrogen-rich medium (i.e., Basal medium and Modified Bristol's medium), reaching a biomass concentration of 2-5 g/l. The growth on nitrogen-limiting MBL medium resulted in higher lipid accumulation (20-53%) but slower growth rate. Higher lipid content (40-53%) and higher lipid productivity (67-144 mg/l/d) were obtained under mixotrophic cultivation with all the culture media used. The fatty acid composition of the microalgal lipid comprises over 60-68% of saturated fatty acids (i.e., palmitic acid (C16:0), stearic acid (C18:0)) and monounsaturated acids (i.e., oleic acid (C18:1)). This lipid composition is suitable for biodiesel production.
研究了一种分离的小球藻（Chlorella vulgaris ESP-31）在不同培养基和培养条件下的生长和产脂情况，包括光养生长（NaHCO3 或 CO2，有光）、异养生长（葡萄糖，无光）、光异养生长（葡萄糖，有光）和混合营养生长（葡萄糖和 CO2，有光）。小球藻 ESP-31 在富氮培养基（即基础培养基和改良布里斯托尔培养基）上更喜欢在光养（CO2）、光异养和混合营养条件下生长，生物量浓度达到 2-5 g/l。在氮限制的 MBL 培养基上生长导致更高的脂类积累（20-53%），但生长速度较慢。在使用所有培养基的混合营养培养下，可获得更高的脂类含量（40-53%）和更高的脂类生产力（67-144 mg/l/d）。微藻脂类的脂肪酸组成由 60-68%以上的饱和脂肪酸（即棕榈酸（C16:0）、硬脂酸（C18:0））和单不饱和脂肪酸（即油酸（C18:1））组成。这种脂质组成适合生物柴油生产。
