用于膜蛋白生产的高通量杆状病毒表达系统

High-Throughput Baculovirus Expression System for Membrane Protein Production.

作者信息

Kalathur Ravi C, Panganiban Marinela, Bruni Renato

机构信息

New York Structural Biology Center, New York Consortium on Membrane Protein Structure (NYCOMPS), 89 Convent Avenue, New York, NY, 10027, USA.

出版信息

Methods Mol Biol. 2016;1432:187-202. doi: 10.1007/978-1-4939-3637-3_12.

DOI:10.1007/978-1-4939-3637-3_12

PMID:27485337

Abstract

The ease of use, robustness, cost-effectiveness, and posttranslational machinery make baculovirus expression system a popular choice for production of eukaryotic membrane proteins. This system can be readily adapted for high-throughput operations. This chapter outlines the techniques and procedures for cloning, transfection, small-scale production, and purification of membrane protein samples in a high-throughput manner.

摘要

杆状病毒表达系统因其易用性、稳健性、成本效益和翻译后机制，成为生产真核膜蛋白的常用选择。该系统可轻松适用于高通量操作。本章概述了以高通量方式克隆、转染、小规模生产和纯化膜蛋白样品的技术和程序。