一种用于研究杆状病毒表达系统中桡足类超绿色荧光蛋白（cop-GFP）对昆虫细胞转染表型变化的模型。
A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System.
作者信息
Shokrollahi Narjes, Shahbazzadeh Delavar, Pooshang-Bagheri Kamran, Habibi-Anbouhi Mahdi, Jahanian-Najafabadi Ali, Behdani Mahdi
机构信息
Biotechnology Research Center, Venom and Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran.
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
出版信息
Iran Biomed J. 2016 Jul;20(3):182-6. doi: 10.7508/ibj.2016.03.008. Epub 2016 Oct 31.
BACKGROUND
Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells.
METHODS
The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells.
RESULTS
Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis.
CONCLUSION
The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.
背景
杆状病毒表达系统是用于生产重组蛋白的最具吸引力和强大的真核表达系统之一。需要一种生物标志物来监测昆虫细胞中的转染效率或蛋白质表达水平。
方法
本研究的目的是构建一种编码桡足类超绿色荧光蛋白（copGFP）的杆状病毒表达载体。为此，构建了所得载体并用于转染草地贪夜蛾细胞。
结果
通过荧光显微镜和蛋白质印迹分析证实了昆虫细胞中copGFP蛋白的表达。
结论
copGFP对照杆粒的应用可被视为昆虫细胞转染的合适对照。
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