Sun Daoyang, Zhang Xinguo, Li Shaohua, Jiang Cai-Zhong, Zhang Yanlong, Niu Lixin
College of Landscape Architecture and Arts, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Crops Pathology and Genetic Research Unit, United States Department of Agriculture, Agricultural Research Service, Davis, CA, 95616, USA.
Planta. 2016 Dec;244(6):1185-1199. doi: 10.1007/s00425-016-2576-5. Epub 2016 Aug 2.
The L. regale ATP-binding cassette transporter gene, LrABCF1 belonging to GCN subfamily, functions as a positive regulator of plant defense against Cucumber mosaic virus, Tobacco rattle virus , and Botrytis cinerea in petunia. ATP-binding cassette (ABC) transporters are essential for membrane translocation in diverse biological processes, such as plant development and defense response. Here, a general control non-derepressible (GCN)-type ABC transporter gene, designated LrABCF1, was identified from Cucumber mosaic virus (CMV)-induced cDNA library of L. regale. LrABCF1 was up-regulated upon inoculation with CMV and Lily mottle virus (LMoV). Salicylic acid (SA) and ethylene (ET) application and treatments with abiotic stresses such as cold, high salinity, and wounding increased the transcript abundances of LrABCF1. Constitutive overexpression of LrABCF1 in petunia (Petunia × hybrida) resulted in an impairment of plant growth and development. LrABCF1 overexpression conferred reduced susceptibility to CMV, Tobacco rattle virus (TRV), and B. cinerea infection in transgenic petunia plants, accompanying by elevated transcripts of PhGCN2 and a few defense-related genes in SA-signaling pathway. Our data indicate that LrABCF1 positively modulates viral and fungal resistance.
帝王百合的ATP结合盒转运蛋白基因LrABCF1属于GCN亚家族，在矮牵牛中作为植物抵御黄瓜花叶病毒、烟草脆裂病毒和灰霉病菌的正向调节因子发挥作用。ATP结合盒（ABC）转运蛋白在多种生物学过程（如植物发育和防御反应）中的膜转运中至关重要。在此，从帝王百合黄瓜花叶病毒（CMV）诱导的cDNA文库中鉴定出一个通用控制非阻遏型（GCN）ABC转运蛋白基因，命名为LrABCF1。接种CMV和百合斑驳病毒（LMoV）后，LrABCF1表达上调。水杨酸（SA）和乙烯（ET）处理以及冷、高盐和创伤等非生物胁迫处理增加了LrABCF1的转录丰度。LrABCF1在矮牵牛（Petunia × hybrida）中的组成型过表达导致植物生长发育受损。LrABCF1过表达使转基因矮牵牛植株对CMV、烟草脆裂病毒（TRV）和灰霉病菌感染的敏感性降低，同时伴随着SA信号通路中PhGCN2和一些防御相关基因转录本的升高。我们的数据表明，LrABCF1正向调节病毒和真菌抗性。
