Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China, China.
J Virol Methods. 2017 Nov;249:69-75. doi: 10.1016/j.jviromet.2017.08.018. Epub 2017 Aug 25.
Viruses commonly infecting lily (Lilium spp.) include: Lily symptomless virus (LSV), Cucumber mosaic virus (CMV) and Lily mottle virus (LMoV). These viruses usually co-infect lilies causing severe economic losses in terms of quantity and quality of flower and bulb production around the world. Reliable and precise detection systems need to be developed for virus identification. We describe the development of a triplex immunocapture (IC) reverse transcription (RT) polymerase chain reaction (PCR) assay for the simultaneous detection of LSV, CMV and LMoV. The triplex IC-RT-PCR was compared with a quadruplex RT-PCR assay. Relative to the quadruplex RT-PCR, the specificity of the triplex IC-RT-PCR system for LSV, CMV and LMoV was 100% for field samples. The sensitivity of the triplex IC-RT-PCR system was 99.4%, 81.4% and 98.7% for LSV, CMV and LMoV, respectively. Agreement (κ) between the results obtained from the two tests was 0.968, 0.844 and 0.984 for LSV, CMV and LMoV, respectively. This is the first report of the simultaneous detection of LSV, CMV and LMoV in a triplex IC-RT-PCR assay. In particular we believe this convenient and reliable triplex IC-RT-PCR method could be used routinely for large-scale field surveys or crop health monitoring of lily.
百合无症病毒（LSV）、黄瓜花叶病毒（CMV）和百合斑驳病毒（LMoV）。这些病毒通常共同感染百合，导致全球花卉和鳞茎产量在数量和质量方面遭受严重经济损失。需要开发可靠和精确的检测系统来进行病毒鉴定。我们描述了一种三重免疫捕获（IC）反转录（RT）聚合酶链反应（PCR）检测方法的开发，用于同时检测 LSV、CMV 和 LMoV。三重 IC-RT-PCR 与四重 RT-PCR 检测方法进行了比较。与四重 RT-PCR 相比，三重 IC-RT-PCR 系统对 LSV、CMV 和 LMoV 的特异性为 100%，对田间样本而言。三重 IC-RT-PCR 系统对 LSV、CMV 和 LMoV 的灵敏度分别为 99.4%、81.4%和 98.7%。两种检测方法的结果之间的一致性（κ）分别为 LSV、CMV 和 LMoV 的 0.968、0.844 和 0.984。这是首次在三重 IC-RT-PCR 检测中同时检测 LSV、CMV 和 LMoV。特别是，我们相信这种方便可靠的三重 IC-RT-PCR 方法可用于大规模田间调查或百合作物健康监测。
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