Association between levobupivacaine and pancuronium. Interference in neuromuscular transmission and blockade in rats.
作者信息
Carvalho Vanessa Henriques, Braga Angélica de Fátima de Assunção, Braga Franklin Sarmento da Silva, Potério Gloria Maria Braga, Santos Filipe Nadir Caparica, Junqueira Fernando Eduardo Féres
机构信息
PhD, Department of Anesthesiology, Faculty of Medical Sciences, Universidade Estadual de Campinas (UNICAMP), Brazil. Intellectual, scientific and design of the study; acquisition and interpretation of data; technical procedures; manuscript preparation and writing, critical revision, supervised all phases of the study.
Associate Professor, Department of Anesthesiology, Faculty of Medical Sciences, UNICAMP, Campinas-SP, Brazil. Intellectual, scientific and design of the study; interpretation of data; technical procedures; manuscript preparation and writing, critical revision, supervised all phases of the study.
出版信息
Acta Cir Bras. 2016 Jul;31(7):486-9. doi: 10.1590/S0102-865020160070000009.
PURPOSE
To evaluate the effects of levobupivacaine on neuromuscular transmission and neuromuscular blockade produced by pancuronium in vitro.
METHODS
Thirty rats were distributed into groups (n = 5) according to the drug used alone or in combination: Group I - levobupivacaine (5 µg.mL-1); Group II - pancuronium (2 µg.mL-1); Group III - pancuronium (2 µg.mL-1) + levobupivacaine (5µg.mL-1). The following parameters were evaluated: 1) amplitude of diaphragmatic response to indirect stimulation, before and 60 minutes after the addition of levobupivacaine and pancuronium alone, and after the addition of levobupivacaine combined with pancuronium; 2) membrane potentials (MP) and miniature endplate potentials (MEPP).
RESULTS
Levobupivacaine alone did not alter the amplitude of muscle response and MP. In preparations previoulsy exposed to levobupivacaine, the block with pancuronium was significantly denser (90.2 ± 15.2%), showing a significant difference (p=0.031) in comparison to the block produced by pancuronium alone (48.9% ± 9.8%). There was a decrease in the frequency and amplitude of MEPPs.
CONCLUSION
Levobupivacaine potentiated the neuromuscular blockade produced by pancuronium, confirming a presynaptic action by a decrease in miniature endplate potentials.
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