Żmigrodzka M, Guzera M, Winnicka A
Pol J Vet Sci. 2016;19(2):407-14. doi: 10.1515/pjvs-2016-0051.
Platelets play a crucial role in hemostasis. Their activation has not yet been evaluated in healthy dogs with a normal and low platelet count. The aim of this study was to determine the influence of activators on platelet activation in dogs with a normal platelet count and asymptomatic thrombocytopenia. 72 clinically healthy dogs were enrolled. Patients were allocated into three groups. Group 1 consisted of 30 dogs with a normal platelet count, group 2 included 22 dogs with a platelet count between 100 and 200×109/l and group 3 consisted of 20 dogs with a platelet count lower than 100×109/l. Platelet rich-plasma (PRP) was obtained from peripheral blood samples using tripotassium ethylenediaminetetraacetic acid (K3-EDTA) as anticoagulant. Next, platelets were stimulated using phorbol-12-myristate-13-acetate or thrombin, stabilized using procaine or left unstimulated. The expression of CD51 and CD41/CD61 was evaluated. Co-expression of CD41/CD61 and Annexin V served as a marker of platelet activation. The expression of CD41/CD61 and CD51 did not differ between the 3 groups. Thrombin-stimulated platelets had a significantly higher activity in dogs with a normal platelet count than in dogs with asymptomatic thrombocytopenia. Procaine inhibited platelet activity in all groups. In conclusion, activation of platelets of healthy dogs in vitro varied depending on the platelet count and platelet activator.
血小板在止血过程中发挥着关键作用。其激活情况尚未在血小板计数正常和偏低的健康犬中进行评估。本研究的目的是确定激活剂对血小板计数正常和无症状血小板减少症犬血小板激活的影响。纳入了72只临床健康犬。将这些犬分为三组。第1组由30只血小板计数正常的犬组成，第2组包括22只血小板计数在100至200×10⁹/L之间的犬，第3组由20只血小板计数低于100×10⁹/L的犬组成。使用乙二胺四乙酸三钾（K3 - EDTA）作为抗凝剂，从外周血样本中获取富血小板血浆（PRP）。接下来，使用佛波醇-12-肉豆蔻酸酯-13-乙酸酯或凝血酶刺激血小板，使用普鲁卡因使其稳定或不进行刺激。评估CD51和CD41/CD61的表达。CD41/CD61与膜联蛋白V的共表达作为血小板激活的标志物。三组之间CD41/CD61和CD51的表达没有差异。凝血酶刺激的血小板在血小板计数正常的犬中比在无症状血小板减少症犬中的活性显著更高。普鲁卡因在所有组中均抑制血小板活性。总之，健康犬血小板在体外的激活情况因血小板计数和血小板激活剂而异。
