Xidian University, Engineering Research Center of Molecular and Neuro Imaging of Ministry of Education and School of Life Science and Technology, 266 Xinglong Section of Xifeng Road, Xi'an 710071, China.
Fourth Military Medical University, State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, 127 Changle Road, Xi'an 710032, China.
J Biomed Opt. 2016 Aug 1;21(8):86001. doi: 10.1117/1.JBO.21.8.086001.
The aim of this article is to investigate the influence of a tracer injection dose (ID) and camera integration time (IT) on quantifying pharmacokinetics of Cy5.5-GX1 in gastric cancer BGC-823 cell xenografted mice. Based on three factors, including whether or not to inject free GX1, the ID of Cy5.5-GX1, and the camera IT, 32 mice were randomly divided into eight groups and received 60-min dynamic fluorescence imaging. Gurfinkel exponential model (GEXPM) and Lammertsma simplified reference tissue model (SRTM) combined with a singular value decomposition analysis were used to quantitatively analyze the acquired dynamic fluorescent images. The binding potential (Bp) and the sum of the pharmacokinetic rate constants (SKRC) of Cy5.5-GX1 were determined by the SRTM and EXPM, respectively. In the tumor region, the SKRC value exhibited an obvious trend with change in the tracer ID, but the Bp value was not sensitive to it. Both the Bp and SKRC values were independent of the camera IT. In addition, the ratio of the tumor-to-muscle region was correlated with the camera IT but was independent of the tracer ID. Dynamic fluorescence imaging in conjunction with a kinetic analysis may provide more quantitative information than static fluorescence imaging, especially for a priori information on the optimal ID of targeted probes for individual therapy.
本文旨在研究示踪剂注射剂量（ID）和相机积分时间（IT）对定量检测 Cy5.5-GX1 在胃癌 BGC-823 细胞异种移植瘤小鼠药代动力学的影响。基于是否注射游离 GX1、Cy5.5-GX1 的 ID 和相机 IT 这三个因素，将 32 只小鼠随机分为 8 组，并接受 60 分钟的动态荧光成像。使用 Gurfinkel 指数模型（GEXPM）和 Lammertsma 简化参考组织模型（SRTM）结合奇异值分解分析对获得的动态荧光图像进行定量分析。通过 SRTM 和 EXPM 分别确定 Cy5.5-GX1 的结合潜力（Bp）和药代动力学速率常数（SKRC）的总和。在肿瘤区域，SKRC 值随着示踪剂 ID 的变化呈现明显趋势，但 Bp 值对此不敏感。Bp 和 SKRC 值均与相机 IT 无关。此外，肿瘤与肌肉区域的比值与相机 IT 相关，但与示踪剂 ID 无关。动态荧光成像结合动力学分析可能比静态荧光成像提供更多的定量信息，特别是对于个体化治疗中靶向探针的最佳 ID 的先验信息。
