Dai Yunpeng, Chen Xueli, Yin Jipeng, Wang Guodong, Wang Bo, Zhan Yonghua, Nie Yongzhan, Wu Kaichun, Liang Jimin
Engineering Research Center of Molecular and Neuro Imaging of Ministry of Education & School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710071, China.
These authors contributed equally to this work.
Biomed Opt Express. 2018 Mar 26;9(4):1859-1870. doi: 10.1364/BOE.9.001859. eCollection 2018 Apr 1.
Dynamic optical data from a series of sampling intervals can be used for quantitative analysis to obtain meaningful kinetic parameters of probe . The sampling schemes may affect the quantification results of dynamic fluorescence imaging. Here, we investigate the influence of different sampling schemes on the quantification of binding potential () with theoretically simulated and experimentally measured data. Three groups of sampling schemes are investigated including the sampling starting point, sampling sparsity, and sampling uniformity. In the investigation of the influence of the sampling starting point, we further summarize two cases by considering the missing timing sequence between the probe injection and sampling starting time. Results show that the mean value of exhibits an obvious growth trend with an increase in the delay of the sampling starting point, and has a strong correlation with the sampling sparsity. The growth trend is much more obvious if throwing the missing timing sequence. The standard deviation of is inversely related to the sampling sparsity, and independent of the sampling uniformity and the delay of sampling starting time. Moreover, the mean value of obtained by uniform sampling is significantly higher than that by using the non-uniform sampling. Our results collectively suggest that a suitable sampling scheme can help compartmental modeling of dynamic fluorescence imaging provide more accurate results and simpler operations.
来自一系列采样间隔的动态光学数据可用于定量分析，以获取探针有意义的动力学参数。采样方案可能会影响动态荧光成像的定量结果。在此，我们通过理论模拟和实验测量数据研究不同采样方案对结合潜能（）定量的影响。研究了三组采样方案，包括采样起始点、采样稀疏性和采样均匀性。在研究采样起始点的影响时，我们通过考虑探针注射与采样起始时间之间的缺失时间序列进一步总结了两种情况。结果表明，随着采样起始点延迟增加，的平均值呈现明显的增长趋势，并且与采样稀疏性有很强的相关性。如果忽略缺失的时间序列，增长趋势会更加明显。的标准差与采样稀疏性呈负相关，与采样均匀性和采样起始时间的延迟无关。此外，均匀采样获得的的平均值显著高于非均匀采样。我们的结果共同表明，合适的采样方案有助于动态荧光成像的房室建模提供更准确的结果和更简单的操作。
