Fang Zhen, Liu Yaowu, Ni Buqing, Chen Xin-Guang, Zhao Liyan, Zhang Fengxiang
Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Jiangsu-China.
Department of Cardiothoracic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing Jiangsu-China.
Anatol J Cardiol. 2016 Sep;16(9):662-666. doi: 10.5152/AnatolJCardiol.2015.6542. Epub 2015 Nov 25.
Previous genome-wide association studies (GWASs) have identified rs6817105-a single nucleotide polymorphism (SNP) on chromosome 4q25-to be associated with the risk of atrial fibrillation (AF) in a European-descent population. We recently demonstrated this association in a large cohort of Japanese ancestry. Our present study was designed to determine this association in the Chinese Han population.
This case-control study included 597 AF cases and 996 AF-free controls, and rs6817105 SNPs were genotyped using the TaqMan allelic discrimination assay. Odds ratios (ORs) and 95% confidence intervals (95%CIs) were calculated in logistic regression models.
The genotype distribution of rs6817105-CC was significantly more frequent in the AF patients than in the controls (p=3.24×10-32). In our study, logistic regression analysis showed a strong association between rs6817105 and the risk of AF (additive model: OR=2.22, 95%CI=1.89-2.61, p=2.33×10-22; dominant model: OR=2.96, 95%CI: 2.16-4.07, p=2.03×10-11; recessive model: OR=2.83, 95%CI=2.27-3.54, p=4.00×10-20). Stratification analyses showed a borderline statistical difference between subgroups of age for the association of rs6817105 with AF risk (p=0.049). However, further interactive analysis indicated no significant interaction between genotype of rs6817105 and age (p=0.178).
Our finding suggested that SNP rs6817105 may be associated with a high significant risk of AF in the Chinese Han population, although more replicative studies of larger sample size are needed to confirm this finding.
既往全基因组关联研究（GWAS）已确定4号染色体4q25上的单核苷酸多态性（SNP）rs6817105与欧洲裔人群的房颤（AF）风险相关。我们最近在一个大型日本血统队列中证实了这种关联。本研究旨在确定中国汉族人群中的这种关联。
这项病例对照研究纳入了597例AF患者和996例无AF的对照，使用TaqMan等位基因鉴别分析对rs6817105 SNP进行基因分型。在逻辑回归模型中计算比值比（OR）和95%置信区间（95%CI）。
rs6817105-CC基因型在AF患者中的分布频率显著高于对照组（p=3.24×10-32）。在我们的研究中，逻辑回归分析显示rs6817105与AF风险之间存在强关联（相加模型：OR=2.22，95%CI=1.89-2.61，p=2.33×10-22；显性模型：OR=2.96，95%CI：2.16-4.07，p=2.03×10-11；隐性模型：OR=2.83，95%CI=2.27-3.54，p=4.00×10-20）。分层分析显示，rs6817105与AF风险关联在年龄亚组之间存在临界统计学差异（p=0.049）。然而，进一步的交互分析表明，rs681,7105基因型与年龄之间无显著交互作用（p=0.178）。
我们的研究结果表明，SNP rs6817105可能与中国汉族人群中AF的高显著风险相关，尽管需要更多更大样本量的重复研究来证实这一发现。
