Delwing-de Lima Daniela, Delwing-Dal Magro Débora, Vieira Cindy Laís Pett, Grola Gislaine Maria Marestoni, Fischer Débora Adriana, de Souza Wyse Angela Terezinha
Departamento de Medicina, Universidade da Região de Joinville- UNIVILLE, Rua Paulo Malschitzki, 10 - Zona Industrial Norte, CEP, 89201-972, Joinville, SC, Brazil.
Departamento de Ciências Naturais, Centro de Ciências Exatas e Naturais, Universidade Regional de Blumenau, Rua Antônio da Veiga, 140, CEP 89012-900, Blumenau, SC, Brazil.
J Biochem Mol Toxicol. 2017 Jan;31(1):1-7. doi: 10.1002/jbt.21830. Epub 2016 Aug 4.
We investigated the in vitro and in vivo effects of arginine (Arg) on thiobarbituric acid-reactive substances (TBA-RS) and on the activities of catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) in renal tissues of rats. We also studied the influence of antioxidants (α-tocopherol plus ascorbic acid) and nitric oxide synthase inhibitor N -nitro-l-arginine methyl ester (l-NAME) on the effects elicited by Arg. Results showed that Arg in vitro (1.5 mM) decreased SOD activity and increased the levels of TBA-RS in the renal medulla. Acute administration of Arg [0.8 g/kg, intraperitoneal injection] decreased CAT activity, increased SOD activity and TBA-RS levels in the renal medulla, and decreased CAT activity in the renal cortex of rats. Most results were prevented by antioxidants and/or l-NAME. Data indicate that Arg causes an oxidative imbalance in the renal tissues studied; however, in the presence of antioxidants and l-NAME, some of these alterations in oxidative stress were prevented.
我们研究了精氨酸（Arg）对大鼠肾组织中硫代巴比妥酸反应性物质（TBA-RS）以及过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）活性的体内外影响。我们还研究了抗氧化剂（α-生育酚加抗坏血酸）和一氧化氮合酶抑制剂N-硝基-L-精氨酸甲酯（L-NAME）对Arg所引发效应的影响。结果显示，体外实验中1.5 mM的Arg降低了肾髓质中SOD的活性并提高了TBA-RS的水平。急性给予Arg[0.8 g/kg，腹腔注射]降低了大鼠肾髓质中CAT的活性，提高了SOD的活性和TBA-RS的水平，并降低了肾皮质中CAT的活性。大多数结果可被抗氧化剂和/或L-NAME阻止。数据表明，Arg在所研究的肾组织中导致了氧化失衡；然而，在存在抗氧化剂和L-NAME的情况下，氧化应激中的一些改变得到了预防。
