Yang Dong-Kun, Kim Ha-Hyun, Choi Sung-Suk, Lee Seong Heon, Cho In-Soo
Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Gimcheon, Korea.
Clin Exp Vaccine Res. 2016 Jul;5(2):169-74. doi: 10.7774/cevr.2016.5.2.169. Epub 2016 Jul 29.
Rabies viruses (RABV) circulating worldwide in various carnivores occasionally cause fatal encephalitis in swine. In this study, the safety and immunogenicity of a recombinant rabies virus, the ERAGS strain constructed with a reverse genetics system, was evaluated in domestic pigs.
Growing pigs were administered 1 mL (10(8.0) FAID50/mL) of the ERAGS strain via intramuscular (IM) or oral routes and were observed for 4 weeks' post-inoculation. Three sows were also inoculated with 1 mL of the ERAGS strain via the IM route. The safety and immunogenicity in swine were evaluated using daily observation and a virus-neutralizing assay (VNA). Fluorescent antibody tests (FAT) for the RABV antigen and reverse transcriptase-polymerase chain reaction (RT-PCR) assays for the detection of the nucleocapsid (N) gene of RABV were conducted with brain tissues from the sows after necropsy.
The growing pigs and sows administered the ERAGS strain did not exhibit any clinical sign of rabies during the test period test and did develop VNA titers. The growing pigs inoculated with the ERAGS strain via the IM route showed higher VNA titers than did those receiving oral administration. FAT and RT-PCR assays were unable to detect RABV in several tissues, including brain samples from the sows.
Our results suggest that the ERAGS strain was safe in growing pigs and sows and induced moderate VNA titers in pigs.
在世界各地各种食肉动物中传播的狂犬病病毒（RABV）偶尔会导致猪的致命性脑炎。在本研究中，对用反向遗传学系统构建的重组狂犬病病毒ERAGS株在家猪中的安全性和免疫原性进行了评估。
给生长猪通过肌肉注射（IM）或口服途径接种1 mL（10(8.0) FAID50/mL）的ERAGS株，并在接种后观察4周。还通过IM途径给3头母猪接种1 mL的ERAGS株。使用每日观察和病毒中和试验（VNA）评估猪的安全性和免疫原性。对母猪尸检后的脑组织进行狂犬病病毒抗原的荧光抗体试验（FAT）和用于检测狂犬病病毒核衣壳（N）基因的逆转录聚合酶链反应（RT-PCR）检测。
接种ERAGS株的生长猪和母猪在试验期间未表现出任何狂犬病临床症状，并且确实产生了VNA滴度。通过IM途径接种ERAGS株的生长猪显示出比口服给药的猪更高的VNA滴度。FAT和RT-PCR检测在包括母猪脑样本在内的多个组织中未检测到狂犬病病毒。
我们的结果表明，ERAGS株在家猪和母猪中是安全的，并在猪中诱导了中等水平的VNA滴度。
