Vengušt Gorazd, Hostnik Peter, Cerovšek Mojca, Cilenšek Polona, Malovrh Tadej
Institute for Breeding and Health Care of Wild Animals, Fishes and Bees, University of Ljubljana, Ljubljana, Slovenia.
Acta Vet Hung. 2011 Mar;59(1):149-54. doi: 10.1556/AVet.59.2011.1.14.
Serum samples of 746 shot wild boars collected throughout Slovenia during the hunting season of 2005/2006 were examined for the presence of antibodies against rabies virus: 541 samples were collected in areas subjected to yearly antirabies vaccination, and 205 samples were collected in areas where preventive antirabies vaccination was not practised. Using a modified enzyme-linked immunosorbent assay (ELISA), in 209 out of 746 sera (28%) the levels of antibodies against rabies virus were higher than 0.5 IU/ml and deemed positive. A total of 173/541 (32%) and 36/205 (18%) samples were positive in the vaccinated and nonvaccinated areas, respectively. Further analysis of 191 out of the 746 samples using the fluorescent antibody virus neutralisation (FAVN) test revealed the presence of antibodies against rabies virus in 122/191 (64%) samples. This is the first extended research reporting that antibodies against rabies virus that originate from preventive oral vaccination targeting the fox population are present in wild boar.
在2005/2006年狩猎季节期间，对斯洛文尼亚各地采集的746份野猪血清样本进行了检测，以确定是否存在抗狂犬病病毒抗体：541份样本采集自每年进行抗狂犬病疫苗接种的地区，205份样本采集自未进行预防性抗狂犬病疫苗接种的地区。使用改良的酶联免疫吸附测定（ELISA）法，在746份血清中的209份（28%）中，抗狂犬病病毒抗体水平高于0.5 IU/ml，被判定为阳性。在接种疫苗地区和未接种疫苗地区，分别有173/541（32%）和36/205（18%）的样本呈阳性。使用荧光抗体病毒中和（FAVN）试验对746份样本中的191份进行进一步分析，结果显示122/191（64%）的样本中存在抗狂犬病病毒抗体。这是首次有广泛研究报告称，野猪体内存在源自针对狐狸群体的预防性口服疫苗的抗狂犬病病毒抗体。
