Castillo Sonsire Fernández, Chovel Mario Landys, Hernández Niurka Gutiérrez, González Lorena Corcho, Blanco Amaya, Hernández Daily Serrano, Medina Mildrey Fariñas, Tito Maydelis Álvarez, Quiñoy José Luis Pérez
Finlay Institute, Havana, Cuba.
Medical Faculty and Institute of Basic and Preclinical Science "Victoria de Girón", University of Medical Sciences of Havana, Havana, Cuba.
Clin Exp Vaccine Res. 2016 Jul;5(2):175-8. doi: 10.7774/cevr.2016.5.2.175. Epub 2016 Jul 29.
In this study, a formulation of Bordetella pertussis proteoliposome (PLBp), diphtheria, and tetanus toxoids and alum (DT-PLBp) was evaluated as a trivalent vaccine candidate in BALB/c mice. Vaccine-induced protection was estimated using the intranasal challenge for pertussis and enzyme-linked immunosorbent assay fvto assess serological responses for diphtheria or tetanus. Both, diphtheria-tetanus-whole cell pertussis (DTP) and diphtheria-tetanus vaccines (DT) were used as controls. Animals immunized with DT-PLBp, PLBp alone, and DTP showed total reduction of CFU in lungs 7 days after intranasal challenge. Likewise, formulations DT-PLBp, DTP, and DT elicited antibody levels ≥2 IU/mL against tetanus and diphtheria, considered protective when neutralization tests are used. Overall, results showed that combination of PLBp with tetanus and diphtheria toxoids did not affect the immunogenicity of each antigen alone.
在本研究中，评估了百日咳博德特氏菌蛋白脂质体（PLBp）、白喉和破伤风类毒素与明矾（DT-PLBp）的配方作为BALB/c小鼠三价疫苗候选物的效果。使用百日咳鼻内攻毒法评估疫苗诱导的保护作用，并使用酶联免疫吸附测定法评估对白喉或破伤风的血清学反应。白喉-破伤风-全细胞百日咳疫苗（DTP）和白喉-破伤风疫苗（DT）均用作对照。用DT-PLBp、单独的PLBp和DTP免疫的动物在鼻内攻毒7天后肺部的菌落形成单位（CFU）总数减少。同样，DT-PLBp、DTP和DT配方诱导的抗破伤风和白喉抗体水平≥2 IU/mL，在使用中和试验时被认为具有保护性。总体而言，结果表明PLBp与破伤风和白喉类毒素的组合不会影响每种抗原单独的免疫原性。
