Pollot Beth E, Corona Benjamin T
Extremity Trauma and Regenerative Medicine, United States Army Institute of Surgical Research, 3698 Chambers Pass, Bldg 3611, Fort Sam Houston, TX, 78234-6315, USA.
Methods Mol Biol. 2016;1460:19-31. doi: 10.1007/978-1-4939-3810-0_2.
Volumetric muscle loss (VML) injury is prevalent in severe extremity trauma and is an emerging focus area among orthopedic and regenerative medicine fields. VML injuries are the result of an abrupt, frank loss of tissue and therefore of different etiology from other standard rodent injury models to include eccentric contraction, ischemia reperfusion, crush, and freeze injury. The current focus of many VML-related research efforts is to regenerate the lost muscle tissue and thereby improve muscle strength. Herein, we describe a VML model in the anterior compartment of the hindlimb that is permissible to repeated neuromuscular strength assessments and is validated in mouse, rat, and pig.
容积性肌肉损失（VML）损伤在严重的肢体创伤中很常见，是骨科和再生医学领域中一个新兴的重点研究领域。VML损伤是组织突然、明显丧失的结果，因此其病因与其他标准啮齿动物损伤模型不同，后者包括离心收缩、缺血再灌注、挤压和冷冻损伤。目前许多与VML相关的研究工作的重点是再生失去的肌肉组织，从而提高肌肉力量。在此，我们描述了一种后肢前侧隔室的VML模型，该模型允许重复进行神经肌肉力量评估，并已在小鼠、大鼠和猪身上得到验证。
